BI 234 LAB FINAL PCC EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
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Course
BI 234
Institution
BI 234
BI 234 LAB FINAL PCC EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
What are the shapes of bacteria?
circular, rhizoid, irregular, filamentous, spindle
what are the basic stains (positive)
Crystal violet, Safranin, methylene blue (stains organism)
what are the Acidic stains (ne...
used to determine the ability of a microbe to produce gelatinases. Gelatinase-positive
organism will liquefy and gelatinase-negative organism will remain solid. Differentiates
S. aureus from S. epidermidis
SIM Medium Test
Sulfur, Indole, and Motility (Indole production by tryptophanase). Differentiate E. coli
from other enterobacteria.
Lysine Decarboxylase Test
detects enzymatic removal of the carboxyl group from lysine
medium becomes more alkaline causing a pH indicator to change color. Purple =
decarboxylation, yellow= fermentation (no decarboxylation), no color change = no
decarboxylation (brown/orange color)
Phenol Red Test
Differential tests for sugar fermentation and gas production (gram- enteric bacteria). 4
tubes: glucose, lactose, mannitol, sucrose. YELLOW below pH of 6.8 and PINK above
pH 7.4
Durham Tube Sugar Fermentation
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