Microbiology WGU D311 - OA Prep Questions and Already Passed Solutions 2024!!
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Course
WGU D311
Institution
WGU D311
Microbiology WGU D311 - OA Prep Questions and Already Passed Solutions 2024!!
What is a Basic Stain?
If the chromophore is the positively charged ion
What is an Acidic Stain?
if the negative ion is the chromophore
What is a negative stain?
A stain that is absorbed by the background but ...
, What is a Basic Stain?
If the chromophore is the positively charged ion
What is an Acidic Stain?
if the negative ion is the chromophore
A
What is a negative stain?
A stain that is absorbed by the background but not by the cells or organisms in the
VI
specimen.
What does a negative stain produce?
TU
An outline or silhouette of the organisms against a colorful background.
What is Gram Stain used for?
It is a differential staining procedure that involves multiple steps to distinguish between
IS
bacteria with different types of cell walls.
How many steps are involved in Gram Stain?
M
Gram Stain involves multiple steps.
O
What is Acid-Fast Staining?
Acid-fast staining is a differential staining technique and is able to differentiate two types
A
of gram-positive cells: those that have waxy mycolic acids in their cell walls, and those
that do not. uses carbolfuchsin as the primary stain.
N
What happens with Acid-Fast Staining?
JP
The waxy, acid-fast cells retain the carbolfuchsin even after a decolorizing agent (an
acid-alcohol solution) is applied. A secondary counterstain, methylene blue, is then
applied, which renders non-acid-fast cells blue.
What is the purpose of using heat in the endospore stain technique?
To push the primary stain (malachite green) into the endospore
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