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PHMY 610 MICRO LAB EXAM 3 QUESTIONS AND ANSWERS
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PHMY 610 MICRO LAB EXAM 3 QUESTIONS AND ANSWERS...
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PHMY 610 MICRO LAB EXAM 3 QUESTIONSAND ANSWERS
how long might it take with traditional blood culture to get the final culture and
susceptibility report
96 hours
when we say get 1 blood culture what does that mean
a set of 2 bottles = 1 blood culture
why are there 2 bottles with 1 blood culture
for aerobic testing and anaerobic testing
what do the blood culture bottles contain and why?
resin that binds any antibiotic that may be present in blood to allow the bacteria
to grow better
why is it important to know when the growth of bacteria begins in culture aka
time to positivity (TTP)
indicates bacterial burden
a high bacterial burden is associated with a _______ TTP (usually)
quicker TTP
what type of bacteria may grow slower than typical organisms and why?
fastidious bacteria since they want specific environments for growth
how long are typical bacterial cultures held for
up to 5 days (95% of bacteria grows in 2 days)
what will gram stain tell us about bacteria
if gram positive or negative and the shape
how long can traditional biochemical analysis for identification of bacteria take
up to 3 days
, what does catalase test distinguish between
streptococci (negative) and staphylococci (positive)
if catalase positive, is it staph. or strep.
staph.
what will be produced if the bacteria is catalase positive
bubbles on the slide
if catalase negative, is it staph. or strep.
strep.
what does coagulase testing distinguish
between the different staphylococci species
what would coagulase positive result mean
staphylococcus aureus
what would coagulase negative result mean
coagulase negative staphylococcus (CoNS)
once growth is detected in the culture bottle, what occurs
sample is plated on agar plates
T/F: if we find out type of bacteria through biochemical pathways or rapid
diagnostics, we DO NOT have to do growth on agar plate
false, still have to get susceptibility
what is the gold standard testing for susceptibility
agar plate
what is there a higher chance of with multiplex PCR
false positive due to common commensals in the body - too sensitive
what might blood culture be contaminated with and be seen with multiplex PCR
coagulase negative staphylococcus
how fast might we get results with multiplex PCR
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