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Cell Biology 2B03 - Midterm 1 Exam Questions and Answers Problems in normal protein folding mechanisms lead to? - ANSWER-fibril formation Examples of 3 general problems in protein folding? - ANSWER-1) mutation 2) old age 3) prion infection examples of 4 more specific problems leading to fibri...

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  • November 3, 2024
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Cell Biology 2B03 - Midterm 1 Exam

Questions and Answers


Problems in normal protein folding mechanisms lead to? - ANSWER✔✔-fibril formation


Examples of 3 general problems in protein folding? - ANSWER✔✔-1) mutation


2) old age


3) prion infection


examples of 4 more specific problems leading to fibril formation - ANSWER✔✔-1) Genetic amyloid-

related diseases: single change in nucleotide lead to changed AA and unstable intermediates


2) Protein fragments do not fold into proper globular form


3) Breakdown of chaperone system


4) Infection by misfolded fibril structures


examples of prion-based diseases - ANSWER✔✔-Mad Cow disease


Creutzfield-Jacob disease


Fatal Familial Insomnia


Hereditary diseases related to improper protein folding - ANSWER✔✔-Alzheimer's disease


Tay-Sachs


Cystic Fibrosis

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, Copyright © KAYLIN 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH NOVEMBER, 2024


UPR in response to? - ANSWER✔✔-Unfolded Protein Response; accumulation of misfolded proteins


Prolonged UPR =? - ANSWER✔✔-Cell apoptosis (death)


Modes of prion acquisition? - ANSWER✔✔-1) Spontaneous


2) Hertiable


3) Infectious


prions = - ANSWER✔✔-abnormal form of normal protein


Biochemical properties of prions? - ANSWER✔✔-rich in beta sheets, insoluble, aggregation, PK

(antibody) resistant


Clinical features of prion diseases - ANSWER✔✔-dementia, ataxia, visual deficits, akinetic mutism


Effect of abnormal proteins on normal proteins? Result? - ANSWER✔✔-Induce misfolding into disease

form. Amyloid, insoluble fibrous protein aggregates


PrP(Sc) accumulates where in diseased cells? Impairs? - ANSWER✔✔-Recycling endosomes (some in

Golgi Apparatus too). Impairs Golgi trafficking.


Molecular chaperones - ANSWER✔✔-bind to short segments of another protein to assist in protein

folding, prevent aggregation and degradation


Chaperonins - ANSWER✔✔-Chamber-like proteins where proteins can be sequestered and fold in

insolation from the enviroment


Number of different polypeptide sequences - ANSWER✔✔-20^n


Proteins fold ______ to form _________ - ANSWER✔✔-spontaneously, random-coil (statistical coil)


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