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NDSU Microbiology Lab Final Exam Study Guide
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NDSU Microbiology Lab FinalExam Study Guide
Primary stain - Answer crystal violet- purple dye
What is the purpose of IMViC testing? - Answer To distinguish between fecal and non
fecal coliforms?
Selective media - Answer grow specific microorganisms and withhold others
What are non-iodizing radiation - Answer visible light and ultraviolet light
ultraviolet radiation damages bacteria by introducing.... - Answer thymine dimers
Growth media - Answer essential nutrients needed to grow microbes in lab
From exercise 11, where we inoculated glucose broth tubes (containing bromcresol
purple as a pH indicator) with bacteria. Describe the results for typical thermophile and
mesophile - Answer Mesophile will be purple at 55c and Thermophile will be yellow at
55c
Nutrient agar - Answer nutrient broth with added sugar to make it solid
Tempered agar - Answer melted and cooled agar
The planting of Serratia Marcescenes onto agar containing a low level of streptomycin
(NAS) resulted in organisms that developed a resistance to the antibiotic by... - Answer
Random mutations
What does it mean when an organism cant grow in the presence of oxygen - Answer it is
obligate anaerobe
_____ bacteria have high lipid content in their cell walls and retain the carbol fushin stain
even after washing with acidifed- alcohol - Answer Acid Fast
Acid fast stain is important because it diagnosis's ... - Answer Tuberculosis
The gram stain differentiates between bacteria based on the composition of their: -
Answer Cell wall
Acid fast bacteria stain what color - Answer Red
What does alpha hemolysis look like - Answer Green blob
What does beta hemolysis look like - Answer In the picture it appears yellows streaks
What does gamma hemolysis look like - Answer White/ clear streaks
, Looking at a mannitol salt agar plate, which side would show the growth of S. aureus?
And how do you know?? - Answer The yellow side because of the selective media.
Fermentation = yellow
Why are multi drug resistant bacteria an important healthcare concern? What can we do
to combat the problem? - Answer B/C bacteria is becoming resistant fast. Helps if you
listen to your doctor. Take as prescribed. Dont over use. Do not stop medication early
What type of agar is nutrient agar - Answer Growth medium
What type of agar is nutrient agar with streptomycin - Answer Selective
What type of agar is mannitol salt agar - Answer Selective and differential
What type of agar is blood agar - Answer Differential
Which organism produces yellow colonies when grown on nutrient agar and is observed
as cocci in a sarcinae arrangement under the microscope? - Answer M. Luteus
Which of the following is true regarding molds? 1) they are prokaryotic
2) some produce antibiotics
3) they do not cause disease
4) they are non-filamentous - Answer They produce antibiotics
Which technique would you choose to separate microorganisms found in a mixed
specimen? - Answer A streak plate
What components are needed to make nutrient agar? - Answer Distilled water,
nutrients, and agar.
If 5 grams of agar are used to make 1L of N. agar, how many grams are needed for 200
mL of the same medium? - Answer .5 grams.
Pure Cultures - Answer A single microbial species
What is the difference between nutrient agar and nutrient broth? - Answer Broth is
liquid, agar is solid.
Stock cultures - Answer cultures that are maintained for study and reference
What does a positive test of nitrate reduction look like - Answer goes from yellow to Red
What media is differential/ selective an used for detecting coliforms - Answer eosin
methylene blue agar
Isolated colony - Answer pure cultures from a single parent cell
Inoculate - Answer introduce microorganisms into culture medium
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