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BIOCHEMISTRY LAB REPORT 4 2023
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BIOCHEMISTRY LAB REPORT 4 2023/2024|PORTAGE LEARNING|WITH PROCEDURES,
EXPLANATION AND FINAL RESULTS.HIGHLY
VERIFIRD Experiment #: Lab 4 Title: Chromatography
of molecules.
Biuret, Xanthoproteic, Millon's, Ninhydrin - ANSWER Four Test that indicates
presence of Protein
Biuret Test - ANSWER used for identification of proteins and protein estimation
(peptide bonds)
Biuret Test - ANSWER this test uses a drop of aqueous copper sulfate
Biuret Test - ANSWER Positive: Violet
Biuret - ANSWER the chemical product formed when urea is heated to 180 degrees
celcius.
Biuret Test - ANSWER Positive: Nuts, Egg white, Milk, Meat
Negative: Bread
Xanthoproteic test - ANSWER this test indicates presence of proteins (aromatic
amino acids)
Xanthoproteic test - ANSWER this test uses a drop of nitric acid
Nitric Acid - ANSWER this is used for nitration
Residue - ANSWER the loss of elements of water when one amino acid is joined to
another.
Xanthoproteic test - ANSWER Positive: Yellow
(can become orange when an alkaline substance is added)
Xanthoproteic test - ANSWER Positive: Egg albumen, Casein
Negative: Distilled water, Phe, Trp, Gln
Millon's test - ANSWER this test indicates presence of proteins (tyrosine)
Millon's test - ANSWER given by proteins containing phenolic amino acids.
Millon's test - ANSWER Gelatin does not give this test.
, Millon's Test - ANSWER First, white precipitate is formed then it becomes red, upon
boiling.
Millon's test - ANSWER Positive: Phe, Tyr, Egg albumen
Negative: Distilled water, Starch
Ninhydrin test - ANSWER indicates presence of proteins and amino acids.
Ninhydrin solution - ANSWER this solution is prepared by dissolving 0.1 gm of
ninhydrin in about 100 mL of distilled water
Ninhydrin solution - ANSWER this solution is unstable and can only be kept for two
days.
Ninhydrin test - ANSWER Postive: deep blue to violet, pink sometimes red
Ninhydrin Test - ANSWER Positive: Glycine, Gelatin, Tyr, Egg albumen, Casein,
Proline
Mercuric sulfate - ANSWER the phenolic group of tyr of proteins reacts with what in
the presence of sodium nitrite and sulfuric acid.
Amino acids - ANSWER building blocks of protein
Amino acids - ANSWER this is composed of an amino group, carboxyl group, and a
side chain.
Peptide bond - ANSWER chemical bond that forms between the carboxyl group of
one amino acid and the amino group of another.
Denatured protein - ANSWER a protein that has lost its natural structure due to
external factors.
Invertase - ANSWER enzyme that is widely found in nature; catalyzes the hydrolysis
of sucrose into fructose and glucose.
Enzymes - ANSWER biological catalysts that accelerates chemical reactions by
lowering the activation energy required.
Enzyme activity - ANSWER it depends on the enzyme's three-dimensional structure.
pH - ANSWER it affects the ionization of amino acids at the active site.
Temperature - ANSWER it impacts molecular motion
Strawberries - ANSWER this fruit is an excellent source of DNA because they are
easy to pulverize and already contain enzymes that help to break down cell walls.
Octoploid genome - ANSWER strawberries has this genome which causes more
DNA to be isolated.
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