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BMS 602 Exam 3 Questions and Answers 100% Solved | Graded A+

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BMS 602 Exam 3 Questions and Answers 100% Solved | Graded A+ Which organism does MacConkey agar select for, and how does it work? ** - MacConkey is a selective and differential media, that is selective for species of Enterobacteriaceae. * This agar will differentiate between Enterobacteriaecea...

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  • October 30, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BMS 602
  • BMS 602
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BMS 602 Exam 3 Questions and

Answers 100% Solved | Graded A+


Which organism does MacConkey agar select for, and how does it work? **

- ✔✔MacConkey is a selective and differential media, that is selective for

species of Enterobacteriaceae.

* This agar will differentiate between Enterobacteriaeceae based on their

ability to ferment lactose.



MAC inhibits growth of : GRAM POSITIVE organisms through the use of

bile salts and crystal violet.



If an organism ferments lactose (E. coli), the colony pH will drop below 6.8,

and turn pink or red. If an organism does not ferment lactose, the colonies

will remain white/colorless.

Which organisms are TSI slants differential for? How does it work? -

✔✔TSI (triple sugar iron) agar differentiates between species of

, ©NINJANERD 2024/2025. YEAR PUBLISHED 2024.
Enterobacteriaceae on their ability to reduce sulfur and ferment

carbohydrates.



This agar contains:

lactose

sucrose

a small amount of glucose

ferrous sulfate

the pH indicator phenol red



If an organism can ferment any of the sugars present, the medium will turn

yellow. Gas production will collect in the bottom of the tube and push the

agar up. (E. coli ferments all three and produces sulfide gas)



GLUCOSE FERMENTERS: red top, yellow bottom

SULFUR REDUCERS: iron sulfide appears as black precipitate, sometimes

obscures results.

** See slides for specific TSI slant results.

List the results of inocculation on a TSI slant for the following organisms:

*** - ✔✔

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