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K101 LAB QUESTIONS
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K101 LAB QUESTIONS Q&AThe ATP made during anaerobic fermentation is generated by which of the following? -
(correct answer)substrate-level phosphorylation
In the absence of oxygen, yeast cells can obtain energy by fermentation, resulting in the
production of: - (correct answer)ATP, CO2, and ethanol.
During aerobic respiration, H2O is formed. Where does the oxygen atom for the
formation of the water come from? - (correct answer)oxygen (O2)
During cellular respiration in germinating pea seedlings (or other aerobic organisms),
acetyl CoA accumulates in which location? - (correct answer)mitochondrial matrix
Which of the following metabolites enters the citric acid cycle and is formed, in part, by
the removal of CO2 from a molecule of pyruvate? - (correct answer)acetyl CoA
Carbon dioxide (CO2) is released during which of the following stages of cellular
respiration: - (correct answer)Acetyl CoA formation *AND* the citric acid cycle
The primary role of oxygen in cellular respiration is to ___ - (correct answer)serve as an
acceptor for electrons and hydrogen, forming water
The enzyme that facilitates transfer of protons (H+) from the mitochondrial inner
membrane space to the mitochondrial matrix is - (correct answer)ATP synthase ???
Which of the following processes occur regardless of whether oxygen (O2) is present? -
(correct answer)glycolysis
, Yeast cells produce ethanol from pyruvate to do which of the following: - (correct
answer)regenerate NAD+ to continue glycolysis
Which enzymes do bacteria produce naturally as a defense against foreign DNA
injected into the bacteria by a virus? - (correct answer)restriction enzyme
Shown here is a picture of an agarose gel. An EcoR1 digest of a circular plasmid called
pGlo2 was loaded into the first well and run through the gel. Based on the fragment
sizes shown, what is the total size of the pGlo2 plasmid, in base pairs (bp)? - (correct
answer)
After transformation of E. coli with pGLO, why are the cells grown in LB broth for 20
minutes before plating on Amp plates? - (correct answer)There is a phenotypic lag of
~20 minutes before the b-lactamase gene for Amp resistance can be expressed
The "araC" gene on the pGLO plasmid becomes active in the presence of - (correct
answer)arabinose
In the pGLO lab, how could you confirm that a transformed cell was expressing the bla
gene (beta-lactamase) ? - (correct answer)
The bla gene on the pGLO plasmid allows the cells to - (correct answer)
The Ori gene on the pGLO plasmid allows the plasmid to - (correct answer)replicate
E. coli should not grow on the -pGLO LB/amp plate because - (correct answer)the
bacteria did not receive the bla resistance gene
The procedure that helps the E. coli take up the plasmid during the transformation -
(correct answer)heat shocking at 42 degrees for 2 minutes
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