BIOL 301 Exam 1 Study Guide with Complete Solutions
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Course
BIOL 301
Institution
BIOL 301
BIOL 301 Exam 1 Study Guide with
Complete Solutions
Griffith - Ans:-injected mice with bacteria, discovered concept of transformation
Sia & Dawson - Ans:-repeated Griffith's experiment in vitro, proved that mice had nothing to do with
transformation
Avery, MacLeod, and McCarty - Ans:-used DNas...
Taylor, Woods, and Hughes - Ans:✔✔-demonstrated semiconservative replication in eukaryotic cells by
using 3H-thymidine in root tips; used autoradiography to determine radioactivity of chromosomes
John Cairns - Ans:✔✔-discovered origin of replication in E.coli
prokaryotic origin of replication - Ans:✔✔--one per chromosome
-245 nt pairs long
-13bp sequence in 3 tandem repeats, A:T rich
-9bp sequence repeated 4 times (TTATNCANA)
origin of replication in yeast - Ans:✔✔-ARS; 50bp long and includes ATTTATPuTTTA
eukaryotic origin of replication - Ans:✔✔-multiple origins in one molecule
Inman and Schnos - Ans:✔✔-used A:T rich clusters as markers to demonstrate that replication of lambda
chromosome is initiated at a unique origin and proceeds bidirectionally
Arthur Kornberg - Ans:✔✔-demonstrated in vitro synthesis with purified DNA polymerase I
main replicase in E.coli - Ans:✔✔-DNA pol III
leading strand - Ans:✔✔-5-3
lagging strand - Ans:✔✔-overall 3-5
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