BIO 353 EXAM STUDY QUESTIONS
AND ANSWERS
Filopodia contain receptors that sense the environment and...
(a) all filopodia stop moving when the cell is about to extend a lamellpodium
(b) as a first step in walking the lamelipodium forms before the filopodium
(c) a lamellipodium extends only from...
Contact inhibition is a subset of ________. - Ans:✔✔-anchorage dependence for growth.
What limits the growth of a tumor? - Ans:✔✔-invasion by capillaries.
When the 1mm2 tissue fragment is place in the Erlenmeyer flask to loosen the cells in the tissue
(primary explant), what were the components of the medium? - Ans:✔✔-PBS made 0.5% with trypsin
and 3mM with MgCl2.
What happens in primary culture? - Ans:✔✔-Some cells walk out of chunks and adhere to the plate.
Some cells escape but don't adhere to the plate.
Some cells die.
(ESSAY) Explain two methods of making a cell line in detail? Assume in this answer you are starting with
the end of secondary culture moving into making up the cell line. - Ans:✔✔-(ESSAY)
(ESSAY) What are the limitations of cell culture technology? - Ans:✔✔-(ESSAY)
(T/F) Peroxisomes likely originated from endosymbiosis. - Ans:✔✔-false.
(T/F) Peroxisomes increase the fitness of the primitive proto-prokaryote by removing toxic oxygen in the
ancient earth. - Ans:✔✔-true.
(T/F) The endomembrane system supposedly resulted in the formation of the nuclear envelope,
endoplasmic reticulum, Golgi apparatus and primary lysosomes. - Ans:✔✔-true.
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