Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed
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Restriction Enzymes and DNA sequencing
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Restriction Enzymes And DNA Sequencing
Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed
restriction enzymes - Answers enzymes that can cut DNA at specific sequences
What is another name for restriction enzymes? - Answers molecular scissors
who discovered them and how? - Answers Arber and Lynn
were st...
Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed
restriction enzymes - Answers enzymes that can cut DNA at specific sequences
What is another name for restriction enzymes? - Answers molecular scissors
who discovered them and how? - Answers Arber and Lynn
were studying bacteria phages which are viruses that affected bacteria
discovered that there were special enzymes that would recognize phage and viral DNA and cut it up to
help protect the bacteria
restricting growth viruses
endonucleases - Answers in hot nucleic acid. cut at internal areas/location
exonucleases - Answers cut DNA at ends of the molecule
not restriction enzyme
Blunt end cutter - Answers when restriction enzyme cuts the DNA at same location in both strands
Staggered end cutter - Answers cut the DNA at 2 different places forming puzzle pieces
Restriction Digest - Answers cutting DNA with restriction enzymes
Linear DNA - Answers # of retraction fragments= # of cuts + 1
adds to the size or original DNA
draw figure on the gel
Circular DNA - Answers # of restriction fragments= # of cuts
O.R.I - Answers site were replication begins
reference point
Gene Cloning - Answers technique that allows you to generate multiple copies of a gene. all identical to
the original cell
when was the first human gene cloned? - Answers 1986
Clone - Answers Greek work for Klone meaning twig
group of cells that ate identical to the original cell
What are the ingredients of Cloning? There are 5 - Answers 1) gene of interest
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