IMM EXAM QUESTIONS WITH ALL CORRECT ANSWERS
Once you complete addition of samples and standards to the plate, where do you store the plate and for how long?
a. refrigerator for 1 hour
b. room temperature for 24 hours
c. freezer for 1 hour
d. room temperature for 2 hours
e. none of the abov...
IMM EXAM QUESTIONS WITH ALL
CORRECT ANSWERS
Once you complete addition of samples and standards to the plate, where do you store
the plate and for how long?
a. refrigerator for 1 hour
b. room temperature for 24 hours
c. freezer for 1 hour
d. room temperature for 2 hours
e. none of the above - Answer- d. room temperature for 2 hours
What is the best indication that your ELISA was properly conducted?
a. negative control produces no color
b. samples are all blue
c. standard curve has an incremental color intensity
d. untreated samples are clear
e. none of the above - Answer- c. standard curve has an incremental color intensity
The detection antibody in the ELISA system used in this course recognizes?
a. TNF- α
b. IgG
c. GM-CSF
d. CD8
e. all of the above - Answer- a. TNF- α
What component of the ELISA system does the enzyme bind to?
a. capture antibody
b. albumin
c. detection antibody
d. biotin on the detection antibody
e. folic acid on the detection antibody - Answer- d. biotin on the detection antibody
The coating buffer is used to adhere
a. the detection antibody to the plate
b. the enzyme to the plate
, c. cytokine to the plate
d. capture antibody to the plate
e. none of the above - Answer- d. capture antibody to the plate
Once the capture antibody has adhered to the plastic the next step is to
a. discard the capture antibody solution and add the samples
b. discard the capture antibody solution, wash and block with albumin
c. discard the capture antibody solution, wash and block with detection antibody
d. discard the capture antibody solution, wash and block with samples
e. any of the above - Answer- b. discard the capture antibody solution, wash and block
with albumin
What volume from the detection antibody stock tube will you use to prepare a working
solution of detection antibody at 2µg/mL? (Suggested total volume to be prepared:
12mL and antibody stock concentration is 2 mg/mL)
a. 120 µL
b. 1200 µL
c. 12 mL
d. 12 µL
e. none of the above - Answer- d. 12 µL
Which of the following are characteristics of an antibody
a. comprised of 2 heavy chains and 2 light chains
b. chains have a variable and a constant portion
c. chains are held together through disulfide bonds
d. all of the above - Answer- d. all of the above
Light chains can be
a. κ or λ
b. have a variable portion
c. are attached to another light chain through disulfide bonds
d. a and b
e. all of the above - Answer- d. a and b
The variable portion of a chain
a. binds to antigens
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