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Microbiology 210- Lab midterm exam with complete solutions.
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Microbiology 210- Lab midterm exam with complete solutions.
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Microbiology 210- Lab midterm exam withj j j j j
complete solutions
j j j
Define jwhat jmicroorganisms jare. j- jANSWER✓✓-organisms jthat jare jtoo jsmall jto
jsee jwith jthe jhuman jeye.
Compare jand jcontrast jthe j3 jdomains jof jlife. j- jANSWER✓✓-3 jdomains: jArchaea,
jBacteria, jEukaryea. j
Eukarya- jcontain ja jdistinct jnucleus jsurrounded jby ja jlipid jmembrane
Archaea- jno jmembrane jbound jnucleus, jtypically jfound jin jextreme
jenvironments
Bacteria- jno jmembrane jbound jnucleus
1. jWhat jare jdifferent jtypes jof jmicroscopy? j- jANSWER✓✓-bright jlight
jmicroscopy- jlight jrays jpass jthrough jthe jorganism jand jlenses jare jused. j
Dark jfield jmicroscope- jlight jspecimen jviewed jagainst ja jdark jbackground
Phase jstained jmicroscope- jview jorganelles jof jliving jorganisms
Florecence- jused jin jmedical
Electron jmicroscope- juses jelectrons
1. jExplain jthe jdifference jbetween jthe jresolving jpower jand jthe jmaginifying
jpower jof jthe jmicroscope. j- jANSWER✓✓-The jmagnifying jpower jis jthe jnumber
jof jtimes jthe jimage jhas jbeen jmagnified, jand jthe jresolving jpower jis jthe jability
jto jseparate jbetween j2 jclosely jplaced jobjects.
1. jAdvantage jof ja jpar jfocal jmicroscope? j- jANSWER✓✓-The jobjectives jcan jbe
jinterchanged jwithout jchanging jthe jfocus jgreatly. jOnly jthe jfine jadjustment
jknob jneeds jto jbe jused jto jrefocus jthe jimage.
1. jWhy jis joil jnecessary jon jthe j100x jobjective? j- jANSWER✓✓-Most jof jthe jlight
jpassing jthrough jthe jspecimen jwould jbe jrefracted jand jwould jnot jtravel jup jthe
jobjective jlens. jOil jhas jthe jsame jreactive jindex jof jglass jso joil jminimizes jthe
jamount jof jlight jlost jgoing jthrough jthe jlens.
1. jprocedures jfor jcaring, jtransporting, jand jusing ja jmicroscope j- jANSWER✓✓-
1. jgrip jmicroscope jarm jand jcarry jbase jwith janother jhand j
2. jadjust jheight jso jit jcan jbe jlooked jthrough
plug jit jin
, 3. jclean jocular jlens juse
1. j5 jpossible jreasons jwhy jan jobject jmay jbe jhard jto jfind jon ja jmicroscope. j-
jANSWER✓✓-Objective jlens jmay jbe jtoo jlow jin jpower, jnot jfocused, jlight jtoo
jdark jor jbright, jmight jbe jusing jthe j100x jwithout joil, jslide jmay jneed jto jbe
jstained.
1. jWhat jto jdo jif jyou jcan jfind jspecimen jon j4x, j10x jand j100 jx jobjective, jbut
jcan't jfind jit jon j100 jx jobjective. jWhat jto jdo? j- jANSWER✓✓-To jswitch jback jto
j40 jx, jwipe joil joff jslide jand j100x jobjective jfirst.
1. j6 jsteps jbefore jputting jmicroscope jaway? j- jANSWER✓✓-1. jClean jobjectives
jwith jkim jwipe jmoistened jwith jethanol
2. jlower jstage
3. jplace jlowest jobjective j(4x) jpointing jdownwards
4. jturn jlight jdown jand jturn jmicroscope joff
5. jwrap jup jcord jneatly
6. jplace jin jappropriate jcabinet
2. jQualities jof jeukaryotic jmicroorganisms j- jANSWER✓✓-larger jand jmore
jcomplex, jbut jcontain jprokaryotes, jwhich jbecame jorganelles
2. jendosymbiotic jtheory j- jANSWER✓✓-Ancestors jof jmitochondria jand jplastids
jwas jprokaryotes jthat j
came jto jlive jin ja jhost jcell.
2. jFungi jTerminology j- jANSWER✓✓-hyphae- jfilaments j
septae- jcross jwall jthat jdivide jhyphae
2. jmolds j- jANSWER✓✓-Multicellular, jfilamentous jhyphae, jasexual jor jsexual
jreproduction jvia jspores.
2. jHyphae jtypes j- jANSWER✓✓-arial- jgrow jupwards
Vegitative- jgrow jlow jand jflat jon jthe jsurface
rhyzoidal- jgrow jall jthroughout jthe jmedium
2. jproperties jof jmedia jused jto jgrow jmold j- jANSWER✓✓-High jsugar
jconcentration jand ja jpH~ j6.5 j(slightly jacidic jprevents jbacteria jgrowing)
2. jDescribe jwhy jactophenol jCotton jBlue jStain jis jused jwhen jviewing ja jfungal
jspecimen junder ja jmicroscope. j- jANSWER✓✓-It jkills jand jpreserves jthe
jorganisms, jblue jstains jthe jchitin jin jthe jcell jwall.
2. jHow jdo jyeasts jreproduce jasexually? j- jANSWER✓✓-budding
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