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CMMB403 Final Exam Questions and Answers 100% Solved

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CMMB403 Final Exam Questions and Answers 100% Solved 72 hours (8 cells) (BEFORE BLASTOCYST STAGE) - what is the human MZT Mid-blastula transition/maternal-zygotic transition - when the transcription of zygotic genes begins and maternal mRNAs are degraded, the cell cycle lengthens, gap stages ...

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  • October 20, 2024
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CMMB403 Final Exam Questions and

Answers 100% Solved


72 hours (8 cells) (BEFORE BLASTOCYST STAGE) - ✔✔what is the

human MZT

Mid-blastula transition/maternal-zygotic transition - ✔✔when the

transcription of zygotic genes begins and maternal mRNAs are degraded,

the cell cycle lengthens, gap stages are added (before is only M and S) and

the synchronicity of cell division is lost. - embryo runs on maternal

resources (mRNAs and proteins in the egg @ time of fertilization) until this.

is when maternal effect genes are turned off.

gap genes - ✔✔organize cells into groups of segments along anterior-

posterior axis - are activated OR repressed by MEG products expressed

from the zygotic genome (after MZT) and have broad expression domains -

divide embryo into regions about 2 segments wide - when lose function

causes large gaps in embryo where multiple segments lost, missing several

segments in a row - they have 1 or 2 broad stripes of expression and define

,©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.

boundaries in the early embryo - are ZYGOTICALLY expressed - ex:

Kruppel

maternal effect genes - ✔✔A gene that, when mutant in the mother, results

in a mutant phenotype in the offspring, regardless of the genotype -

mRNAs deposited in oocyte set up early polarity translated at fertilization -

dads genes do not matter for this - create the anterior/posterior axis

(cytoplasmic polarity) through bicoid and nanos that form morphogen

gradients possible due to syncytium and are incorporated into cells during

cellularization

pair-rule genes - ✔✔organize cells into individual segments/ divide the

embryo into segment-sized units along the anterior/posterior axis - are

activated OR repressed by products of gap genes - helps to define the

spatial domains of the homeotic genes - expressed at ONSET of

cellularization - have 7 stripes of expression - miss portions of every other

segments and may delete boundaries or reduce segment numbers - when

mutated have half as many segments and result in only 7 stripes instead of

14 and - ex: fushi tarazu and even skipped

homeotic genes - ✔✔Genes that determine basic features of where a body

part is - define the identities of each of the segments - their expression

,©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.

domain is correlated with where they are on the chromosome - are all TFs

that regulate segment specific genes - have vertebrate homologs!

segment polarity genes - ✔✔activated by pair rule gene products after

cellularization, give each individual segment its polarity - have 14 stripes of

expression - when mutated polarity disrupted in each segment (segment

number doesn't change will get 14) - encode for members of shh and wnt

pathways

maternal effect - ✔✔which gene is transcribed BEFORE and upon

fertilization

gap - ✔✔which genes are transcribed after fertilization but before

cellularization and the MZT

pair rule - ✔✔what genes are expressed at the onset of cellularization/just

before - with the MZT

segment polarity - ✔✔what genes are expressed after cellularization and

the MZT

1 or 2 - ✔✔how many stripes of expression do gap genes have (broad)

7 - ✔✔how many stripes of expression do pair rule genes have

14 - ✔✔how many stripes of expression do segment-polarity genes have

, ©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.

Parasegments - ✔✔transient subdivisions that occur in the Drosophila

embryo prior to the formation of segments - are off register by one half from

those of the segments, is made up of the cells in the posterior compartment

of one segment and the anterior compartment of the next segment. the

expression patterns of early segmentation genes are at THESE boundaries

- is the "fundamental unit of embryonic expression"

kruppel - ✔✔gap gene expressed in parasegments 4-6, is repressed by

hunchback, if mutated will be missing several segments in a row (4-6ish)

fushi tarazu (ftz) - ✔✔pair rule gene in drosophila that is expressed in even

# parasegments, if mutated results in no boundaries btw parasegments and

reduced (by half) segment number from 14 to 7

even-skipped (eve) - ✔✔pair rule gene in drosophila that is expressed in

ODD # parasegments, if mutated results in no parasegment boundaries

and reduced segment number from 14 to 7

engrailed (en) - ✔✔segment polarity gene expressed in cells w high [ ] of

either even skipped or fushi tarazu proteins, is a hedgehog pathway

regulator that marks the anterior compartment of each parasegment (which

is also the posterior part of each segment) - this reinforces parasegment

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