Other
Biol G180L Lab 8 Report
- Course
- BIOL G180L
- Institution
- Golden West College
This is a comprehensive and detailed lab report on; lab 8; metabolism. *Essential!!
[Show more]
Content preview
Tran NguyenProfessor Pate
BIO 180 Lab
CRN 60106
04/03/2018
Lab #8: Metabolism
Introduction
Photosynthesis is the process of using sunlight energy to synthesize carbohydrate (CH2O)
from carbon dioxide (CO2), which occurs in the chloroplast where contains chlorophyll
molecules. However, plants continuously undergo cellular respiration, which uses carbohydrate
to produce carbon dioxide. In fact, when plants are exposed to light, there occurs to be both
photosynthesis and cellular respiration; and when plants are in the dark, there occurs only
cellular respiration. Moreover, fermentation is the process of metabolizing glucose into ethanol
(C2H5OH) and carbon dioxide and producing limited amounts of ATP. Finally, paper
chromatography is based on differential adsorption, which is based on variances in solubilities
due to polarity differences, of a solute between the mobile and stationary phases.
Due to the relationship of CO2 and pH, there is a pH indicator called phenol red which
helps determine the increase or decrease of pH in the solutions. As CO2 increases, pH decreases
which means the solution turns more acidic, and vice versa. The neutral color of phenol red is
red; its acidic state’s color is yellow, and the basic state’s color is hot pink.
In exercise 1, some pieces of elodea are put in two solutions of phenol red – one with
light and one without light, to discover the amount of CO2 in the solutions using the
spectrophotometer. In exercise 2, yeast is used to perform the experiment of fermentation, which
releases CO2, and the absorbance values are taken based on the behavior of phenol red with its
relationship to CO2 and pH. In exercise 3, using ascending chromatography, different pigments
found spinach (carotene, chlorophyll a, chlorophyll b, and two xanthophylls) are separated in the
chamber containing 9:1 petroleum ether/acetone.
1
, Tran Nguyen
Photosynthesis/Respiration Results and Discussion
1) What are the colors of the phenol red solutions after 30 minutes?
Control-E solution: yellow orange
Elodea with light: strawberry red
Elodea with no light: slightly orange
Without light
With light
Control-E
Figure 1. Phenol Red Solutions of Elodea in Different Environments
2) What are the absorbance values of the following phenol red solutions?
Control-E solution (0 min): 1.18
Control-E solution (30 min): 1.19
Elodea with light (30 min): 0.91
Elodea with no light (30 min): 1.15
2
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller anyiamgeorge19. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77254 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now