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CRISPR Cas-9 Questions With 100% Correct Answers!!
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CRISPR Cas-9 Questions With 100% Correct Answers!!
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Name: Score:26 Multiple choice questions
,Term 1 of 26
How does the process of CAS 9 work on the normal body.
this process begins due to a protein complex known as CAs 1 and Cas2. They identify the
invading viral DNA and then it cuts out a segment of with a specific length from the viral
DNA.
This segment is called a protospacer. This protospacer is then inserted into the front of the
CRISPR array (el segment con los repeated sequences y los target specific sequences)
So, now the bacteria has memory of this bacteriophage infection so, if the bacteriophage
returns the bacteria is armed to defend itself.
an enzyme that can cut DNA
-can be used to target many genes at once
-a big advantage for studying complex human diseases that
-when bacterium detects that there is virus DNA inside it.. it produced 2
types of short RNA.
-one of the RNA has letter sequence that matches the sequence of the DNA
of the invading virus. This is called the guide RNA.
The two RNAs join in/attach a nuclease (an enzyme that can cut DNA) and
create a complex called CAS 9
-So, the DNA of the virus thinks that the RNA strands are normal so the Virus
DNA attaches to the guide RNA. As if it would about to replicate.
-They connect and then the CAS 9 cuts the DNA strands of the virus DNA.
This disables the virus.
, Term 2 of 26
What is CAS 9?
- a RNA called the trans -acting RNA (or tracrRNA) comes and connects with the the crRNA
that was made form the CRISPR array. -links through base pairings.
-then, the protein cas 9 grabs on to the crRNa and the tracrRNa and creates the CAS 9 by
trims it to a smaller size and created the CRISP CAS 9 complex
stands for protospacer adjacent motif sequences
-it is needed for Cas9 to bind target DNA.
-All PAM sequences need to be identified within the genetic region to be targeted
-it helps cut time when trying to find the protospacer to connect
(PAM is 5′ NGG 3′ for SpCas9).
is the complex that used by bacteria to protect themselves from infection of viruses
-It is the nuclease + the 2 types of short RNA strands (One of the strands is the guide RNA)
theyre a protein complex
They identify the invading viral DNA and then it cuts out a segment of with a specific length
from the viral DNA (protospacer) and adds it to the CRISPR sequences
Term 3 of 26
nuclease
would allow the edit dna of any species including humans
-there are the long rna sequences that crispr makes
a protein found in the Cytoplasm
an enzyme that can cut DNA
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