Gel Electrophoresis Quiz Latest Update
Graded A+
What is the role of the agarose gel in gel electrophoresis?
The agarose gel provides a medium through which the DNA or protein fragments can
migrate, allowing for separation based on size.
How does the electric field affect the movement of...
What is the role of the agarose gel in gel electrophoresis?
✔✔The agarose gel provides a medium through which the DNA or protein fragments can
migrate, allowing for separation based on size.
How does the electric field affect the movement of DNA in gel electrophoresis?
✔✔The electric field causes negatively charged DNA molecules to move towards the positive
electrode, facilitating their separation.
What is a DNA ladder, and why is it important in gel electrophoresis?
✔✔A DNA ladder is a mixture of DNA fragments of known sizes, used as a reference to
determine the size of unknown DNA fragments in the gel.
What factors can influence the resolution of DNA bands during gel electrophoresis?
✔✔Gel concentration, voltage applied, and running time can all influence the resolution of DNA
bands during electrophoresis.
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, Why is it necessary to stain the gel after electrophoresis?
✔✔Staining is necessary to visualize the separated DNA or protein bands, as they are not visible
in the gel without a stain.
What is the purpose of a loading dye in gel electrophoresis?
✔✔A loading dye helps track the progress of the electrophoresis and adds weight to the sample,
allowing it to sink into the wells of the gel.
What might happen if you overload a well with too much DNA during gel electrophoresis?
✔✔Overloading a well can lead to distorted bands and poor resolution, making it difficult to
accurately determine the sizes of the DNA fragments.
How can you ensure that the gel is properly prepared before electrophoresis?
✔✔You can ensure proper preparation by measuring the correct amount of agarose, dissolving it
completely, and allowing it to cool before pouring it into the gel mold.
What happens to DNA fragments as they migrate through the gel during electrophoresis?
✔✔Larger DNA fragments move more slowly through the gel matrix, while smaller fragments
move more quickly, resulting in separation based on size.
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