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MCB 2610 Lab Practical Question and answers verified to pass 2024/2025
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MCB 2610 Lab Practical Question and answers verified to pass 2024/2025 MCB 2610 Lab Practical What is the process of contamination control? - correct answer Aseptic technique What are pipettes - correct answer calibrated glass tubes used to measure and dispense small volumes of liquids...

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  • October 17, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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MCB 2610 Lab Practical
What is the process of contamination control? - correct answer ✔Aseptic
technique


What are pipettes - correct answer ✔calibrated glass tubes used to measure
and dispense small volumes of liquids


What color is the bigger vs. smaller micropipettors pipettes? - correct answer
✔Green - bigger
Blue - smaller


What is the purpose of isolating bacteria? - correct answer ✔To establish a
PURE CULTURE, to be able to study to study the physiology and morphology
of a particular bacterial type


What is the quickest and most economical method of bacterial isolation? -
correct answer ✔Streak plate method


What must you use to view items under 100x? - correct answer ✔immersion
oil


What does parfocal mean? - correct answer ✔It means that if you focus the
specimen in one objective, it'll be in approximate focus in all objectives


What is a simple stain? - correct answer ✔Staining procedures that only use
one stain

, What is a direct stain? - correct answer ✔A simple stain that colors the
bacteria


What is a negative stain? - correct answer ✔Simple stain that stains the
background but leaves the bacteria unstained


What is the purpose of heat fixing? - correct answer ✔1. Denaturing bacterial
enzymes to prevent autolysis
2. Enhances the adherence of bacterial cells to microscope slide


In Gram stains, why can't Gram negative bacteria retain the crystal violet? -
correct answer ✔Because Gram negative bacteria have an outer membrane
and a thinner layer of peptidoglycan, which are easily depolarized and can
only retain the counterstain (Safranin-pink)


What are the 3 most common stain? - correct answer ✔Gram, capsule, and
spore stain


What are stains made of? - correct answer ✔Usually salts, a few acids or
bases, charged ions.


The ion that is colored contains a chromophore group


What is it called if the chromophore is a positive ion (like methylene blue)? -
correct answer ✔Basic stain


What is it called if the chromophore is a negative ion? - correct answer
✔Acidic stain

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