CGMBS ch. 11 Detection and Identification of Microorganisms Questions and Answers 2024/2025
4 views 0 purchase
Course
CGMBS
Institution
CGMBS
Salmonella typhi specimen collection
→ initially present in peripheral bloo but not in urine or stool util at least 2 weeks after
infection
Smallpox must be handled only in approved laboratories
→ level 4 containment
When processing whole blood, why is it important to remove homoglobin
�...
→ B-actin, glyceraldehyde-3-phosphate, interferon-y, extrinsic homologous control, human
mitochondril DNA, peptidylprolyl isomerase A
Homologous extrinsic control
→ target-derived control with a non-target-derived sequence insert; control is added to
every sample after nucleic acid extraction and before amplification
Heterologous extrinsic controls
→ non-target-derived controls that are added to every sample before nucleic acid
extraction; second set of primers must also be added to the reaction for the conrol to
be amplified
Heterologous intrinsic ontrols
→ nontarget sequences natrually present in the sample, such as eukaryotic genes in a test
for microorganisms
Reasons for false-negative results on a sample
→ 1. organism may be present, but nucleic acid was degraded during collection,
transport, and or extraction
→ 2. amplification procedures may be inhibited by substances present in the specimen
Sequencing of the DNA region encoding 16S rRNA is performed to determine
→ the evolutionary and genetic relatedness of microorganisms and has driven changes in
microorganism nomenclature
Microbiological identification and epidemiology
→ mass spectrometry of microbial proteins
Bordetella pertussis
1|Page| GradeA+ | 2 0 0 2 5
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller TestTrackers. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.49. You're not tied to anything after your purchase.
