BIOD 171 Portage Learning Exam 3 Questions With Verified Answers
8 views 0 purchase
Course
BIOD 171
Institution
BIOD 171
BIOD 171 Portage Learning Exam 3
Questions With Verified Answers
Define the measurements micrometer and nanometer. - answerA micrometer is one-millionth
of a meter and a nanometer is one-billionth of a meter.
What are the two critical factors that influence your ability to see an object? - answ...
BIOD 171 Portage Learning Exam 3
Questions With Verified Answers
Define the measurements micrometer and nanometer. - answer✔A micrometer is one-millionth
of a meter and a nanometer is one-billionth of a meter.
What are the two critical factors that influence your ability to see an object? - answer✔The
resolution and the contrast. Resolution is the distance between two objects where you can still
see them as separate. Contrast is the difference in light between two objects.
If you wish to increase the amount of light going into a microscope, what part would you adjust?
- answer✔The iris diaphragm.
As light passes through a microscope, what is the last piece that light passes before reaching your
eyes? - answer✔Once light passes through the sample and the objective lens, it passes through
the OCULAR LENS.
How is the total magnification of an object calculated? - answer✔It is calculated by multiplying
the power of the objective and the power of the eyepiece.
What is one limitation of fixing your sample? - answer✔Fixing your sample requires you to
irreversibly kill your sample.
This can make it impossible to determine the cell movement. You could distort the shape and
structure.
Phase-contrast microscopy provides what benefits to imaging? - answer✔Phase-contrast
microscopy provides detailed images without staining.
What is the distinguishing feature of dark field microscopy? - answer✔Dark field microscopy
reflects light off of the specimen at an angle..
Unlike brightfieqld microscope, fluorescence microscopes illuminate samples through what
spectrum? - answer✔The Ultraviolet Spectrum.
What is the primary difference between TEM and SEM? - answer✔The electron passes through
the sample during TEM where as the electron is reflected off the sample during SEM.
Gram staining is based on what basic principle? - answer✔With the basic observation that
different types of bacteria react differently to various dyes.
What is a key determinant in bacteria being Gram-positive? - answer✔Gram-positive have a
thick peptidoglycan layer. Uses crystal violet and iodine. The resulting mixture is a purple
coloration of the cell.
What is the purpose of heat fixing a sample? - answer✔Heat fixing makes sure the samples are
tightly adhere to the glass slide.
What is the primary purpose of a wet mount? - answer✔Wet mounts are often perform to
visualize live cells and to see the motility and behavior of an organism.
The acid-fast stain is most often used to identify what specific microorganism? -
answer✔Mycobacterium tuberculosis is the most common for an acid-fast stain.
What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? -
answer✔60 x 100 = 600x magnification
True or False: A cell that is adherent, flat, and unstained is easily identified using bright field
microscopy. - answer✔False
Flat cells are almost invisible due to the limits of resolution and contrast
Which of the following could be seen clearly by the unaided eye? - answer✔Protozoa with
diameter of 150um and skin cell with diameter of 1500um
Label the following microscope components by matching it with the components provided. -
answer✔1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
This type of microscope is best suited for visualizing GFP, RFP, and YFP proteins -
answer✔Flourescence
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller sirjoel. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.49. You're not tied to anything after your purchase.
