IRENE GOLD MICROBIOLOGY BOARDS FINAL
EXAM NEWEST 2024-2025 ACTUAL EXAM
COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+
Who is the father of pathology, father of Germ Theory (most
famous). Disproved spontaneous generation. Pasteurization of
wine, then milk? - ANSWER- Pasteur
Who came up with washing of hands before delivering baby and
significantly reduced Peurperal Fever. - ANSWER-
Semmelweiss
What are the 4 Koch's postulates? - ANSWER- 1. Find
microorganism in people suffering from the disease
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2. Isolate and grow microorganism in culture
3. Should cause disease in healthy individual when introduced
4. Reisolate microorganism from the inoculated people
Which stain categorizes Streptococcus and demonstrates type of
hemolysis (destruction of RBC)? - ANSWER- Blood agar
______________ stain = mycobacterium - ANSWER- Acid fast
Which type of hemolysis is partial destruction of RBC, green
ring with show, and will see in strep pneumoniae and strep
viridans? - ANSWER- Alpha
Which type of hemolysis is a complete destruction of RBC,
clear ring and seen in strep pyogenes? - ANSWER- Beta
Which type of hemolysis is no destruction of RBC, shows a red
ring, and seen in strep mutans? - ANSWER- Gamma
Which stain is AKA "heated blood agar" or "Thayer Martin
Agar" and checks for Gonorrhea? - ANSWER- Chocolate agar
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Which stain categorizes based on cell wall (Gram + vs. Gram -)
- ANSWER- Gram stain
Gram stain procedures:
______________ = crystal violet or methylene blue (Gram
positive stains purple/blue)
______________ = iodine --> "Mordant"
______________ = acetone or Ethyl alcohol (alcohol wash).
Removes crystal violet from thin walled (gram -) organisms
______________ = saffarin (red/pink color goes into Gram -
organisms) - ANSWER- Primary stain -- fixer -- decolorizer --
counterstrain
Cell sex of life:
_____________ = plasmid DNA transfer via sex pilus. Bestows
antimicrobial resistance
_____________ = bacteriophage (altered virus) transfers genetic
material b/w bacteria
_____________ = direct uptake of free DNA - ANSWER-
Conjugation -- transduction -- transformation
Which governmental agency is not for research, complies
morbidity and epidemiological statistics? - ANSWER- Center
for Disease Control (CDC)
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Which governmental agency monitors soil, air, and water
quality? - ANSWER- Environmental protection agency (EPA)
Which governmental agency regulates health products released
to public? - ANSWER- Food and Drug Administration (FDA)
Which governmental agency is for RESEARCH, not statistics? -
ANSWER- National institute of Health (NIH)
Population terminology:
1. ____________________ = new disease or rapid increase of a
disease in incidence or in geographic range
2. ___________________ = small number of cases in specific
location. Present all the time
3. ___________________ = rapidly affects many persons in a
certain area within a few days or weeks
4. ___________________ = study of the cause of disease
5. ___________________ = # of New cases in given time
period. E.g. How many new cases last year?
6. ___________________ = more than expected # of cases of a
disease worldwide
7. ___________________ = Total # of cases at particular point
in time? E.g. Total cases right now - ANSWER- 1. Emerging
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