BIO353 Exam 2 Questions And Answers With Verified Tests
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Course
BIO353
Institution
BIO353
BIO353 Exam 2 Questions And Answers With Verified Tests
True or False: It is important to be able to repeatedly fractionate the cell. ANS True
True or False: Homogenization buffer can be identical to an intracellular buffer. ANS True
After homogenization the first centrifugation pellets th...
BIO353 Exam 2 Questions And Answers With Verified
Tests
True or False: It is important to be able to repeatedly fractionate the cell. ANS True
True or False: Homogenization buffer can be identical to an intracellular buffer. ANS True
After homogenization the first centrifugation pellets the ________. ANS Nuclei
A sucrose gradient is used to purify ___ ___ away from other ___ ___ ANS plasma membrane,
membrane components
An organic precipitation works because the solvent ________ disrupts the ______ ___________ of
the solute. ANS ethanol, hydrogen bonding
The binding of the signal recognition particle to the nascent peptide _____ protein synthesis. ANS
arrests
The function of the stop- transfer and start-transfer sequences is to make a _____ protein. ANS
transmembrane
True or False: BiP acts as a chaperone ANS True
During vesicular transport, what is the correct sequences of events as a vesicle leaves the organelle?
ANS Triskelion, clathrin, recycle, Rab, SNARE
The subunit of clathrin is _____ ANS Triskelion
The preassembled carbohydrate tree is added on ___ residue. ANS Asparagine
, What is the role of microtubules in membrane transport? ANS Transport vesicles from ER to
Golgi
Can COP I play a role in protein disulfide isomer position? ANS Yes
Does constitutive exocytosis require more signal sequences than regulative exocytosis? ANS No
Does the inside/outside rule apply only to regulative exocytosis? ANS No
True or False: The carbohydrate tree added to proteins targeted to the primary lysosome is added on
an amino acid different from the amino acid where the carbohydrate tree is added to a transmembrane
protein. ANS False
True or False: At the trans face of the Golgi, triskelion assembles into clathrin to cause removal of
the putative primary lysosome. ANS True
Where is the number 6 carbon of mannose exposed?
-in the nucleus
-in the rough endoplasmic reticulum
-in the smooth endoplasmic reticulum
-in the medial phase of the Golgi
-none of the above ANS None of the above
Where does the receptor for mannose-6-phosphate cap the proteins destined for the primary
lysosome? ANS trans face of the Golgi
True or False: A lipase is a protein. ANS True
When does the proton pump that lowers the pH below 5.5 or lower in the forming primary lysosome
start working? ANS after clathrin has been recycled.
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