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560D Clinical Biochem Final Questions and Answers 2024/2025
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A client with diagnosed iron deficiency anemia comes to you because he eats meat based protein and green leafy vegetables but his iron levels have not increased. What other nutrient is needed to properly absorb iron? 1.) Vitamin B12 2.) Folic Acid 3.) Intrinsic factor 4.) Vitamin C  4.)...

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  • October 10, 2024
  • 76
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • 560d clinical biochem final questions and answers
  • a client with diagnosed iron deficiency anemia com

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560D Clinical Biochem Final Questions
and Answers 2024/2025
A client with diagnosed iron deficiency anemia comes to you because he eats meat based

protein and green leafy vegetables but his iron levels have not increased. What other nutrient

is needed to properly absorb iron?




1.) Vitamin B12

2.) Folic Acid

3.) Intrinsic factor

4.) Vitamin C


 4.) Vitamin C


Antacids can reduce the absorption of iron and copper, leading to iron deficiency anemia

because:




1.) Because the antacids bind the metals

2.) Acidic pH increases Fe3+

3.) DMT1 requires co-transport of H+ ions to internalize divalent cations

4.) Stomach acid properly degrades metals

 3.) DMT1 requires co-transport of H+ ions to internalize divalent cations


Which of the following factors can increase likelihood of getting iron deficiency anemia?



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1.) Celiac disease

2.) All of these responses

3.) Heavy menstruation

4.) Deficiency of copper


 2.) All of these responses


Glucose-6-phosphate dehydrogenase is the enzyme that reduces .......(a)....... to ____(b)____

as part of the hexose monophosphate shunt pathway (ie. pentose phosphate pathway). G6PD

deficiency can lead to RBC hemolysis from oxidant stress because ___(b)___ is no longer

available to help recycle glutathione.




1.) (a) NADPH, (b) NADP+

2.) (a) NADH, (b) NADPH

3.) (a) NADP+, (b) NADPH

4.) (a) NAD+, (b) NADH

 3.) (a) NADP+, (b) NADPH


Sickle Cell Anemia is caused by a point mutation leading to a structural instability of the RBC.

What conditions can create increased stress, leading to increased levels of deoxyhemoglobin

and sickle cell crisis?




A: All of these responses

1.) Decreased pH (acidity)


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2.) High altitude

3.) Increased 2,3-Bisphosphoglycerate


 A: All of these responses


Anemia of chronic disease can be caused from prolonged inflammation (IL-6, cytokines,

infection), which increases level of __________________ to sequester iron in tissues so germs

can't use the iron to reproduce.




1.) Vitamin B12

2.) Aconitase

3.) Hepcidin

4.) Folic Acid

 3.) Hepcidin (Is synthesized by the liver and controls iron homeostasis).


Sideroblastic anemia is an X-linked condition where the enzyme called

____________________ is mutated, leading to excess iron in the RBC mitochondria.




1.) Protoporphyrin IX oxidase

2.) NADH methemoglobin reductase

3.) Delta-aminolevulinic acid synthase

4.) Diaphorase I

 3.) Delta-aminolevulinic acid synthase (this is also the enzyme that controls the rate
limiting step in heme synthesis)


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Copper-containing ferroxidases called Hephaestin and __________ convert Fe2+ to Fe3+ so

it can be safely transported by transferrin in the bloodstream

 A: Ferritin (this is an intracellular storage form of iron)


Which transporter assists in uptake of iron from the intestines?




1.) Divalent metal transporter 1 (DMT1)

2.) Ferritin

3.) Ferrireductase duodenal cytochrome b

4.) Transferrin

 1.) Divalent metal transporter 1 (DMT1)


The Bohr effect explains that a decrease in the amount of oxygen associated with hemoglobin

(Hb) due to a reduced affinity of oxygen for Hb is the result of:




1.) Increased pC02 and increased pH

2.) Increased pCO2 and decreased pH

3.) Decreased pC02 and decreased pH

4.) Decreased pC02 and increased pH


 2.) Increased pCO2 and decreased pH


pCO2 (partial pressure of carbon dioxide)




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