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BCH4024 EXAM 4 PRACTICE PROBLEMS
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BCH4024 EXAM 4 PRACTICE PROBLEMS
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BCH4024 EXAM 4 PRACTICE PROBLEMSWhich of the following is incorrect?
a. The origin of replication complex recognizes the origin in G1
b. Cell division cycle 6 and Cdt1 will join the complex in G1
c. 2 DNA pol will join the complex in S phase
d. 3 mini chromosome maintenance will join the complex in G1
e. Cdk and DDK will initiate replication via phosphorylation in S phase
D
D is incorrect as 2 MCM will be added to the complex, as you
need 1 per strand.
Which of the following prokaryotic and eukaryotic equivalents are not matched up
correctly?
a. DNA pol ɑ-primase, primase
b. MCM, helicase
c. PCNA, β sliding clamp
d. RFA, SSBP
e. FEN-1, gyrase
E
D is incorrect as 2 MCM will be added to the complex, as you
need 1 per strand.
If the coding strand has the sequence 5'-CCTAGGAGC-3' what is the RNA sequence?
a. 5'-CCUAGGAGC-3'
b. 5'-GGAUCCUCG-3'
c. 5'-GGATCCTCG-3'
d. 5'-CCTAGGAGC-3'
e. None of the above
A
What is the bacterial promoter consensus sequence?
a. TTCAGA-TAATAT
b. TTGACA-TATAAT
c. TTGAGA-TATAAT
d. TTCAGA-TATAAT
e. TTGCCA-TATAAT
B
,Which of the following is false?
a. The RNA polymerase holoenzyme initiates transcription with the help of the
sigma subunit
b. The closed complex is formed when the holoenzyme binds to the promoter
c. The loss of the sigma subunit forms the open complex
d. NusA replaces the sigma factor after it dissociates
e. Pausing of the RNA polymerase due to secondary structures allows rho to
displace the RNA polymerase from the DNA strand
C
The loss of the sigma factor leads to the formation of the core
enzyme. The open complex is formed when the sigma factor is still attached and there
is
a transcription bubble of about 12-15 bp.
Which histone keeps DNA in the nucleosome?
a. Histone H1
b. Histone H2A
c. Histone H2B
d. Histone H3
e. Histone H4
A
What is one aspect of regulation of the lac operon?
a. The lac repressor binds to the operator in the presence of allolactose
b. O2 at the lac promoter is the highest affinity binding site, while O1 and O3 are low
affinity sites
c. The lacI gene encodes the lac repressor and is upstream the lac operon
d. CRP binds to the lac repressor up stream the promoter
e. None of the above
C
8. Which of the following RNA polymerases is correctly matched with its function?
a. RNA polymerase I; synthesizing rRNA and mRNA
b. RNA polymerase II; synthesizing tRNA
c. RNA polymerase III; synthesizing mRNA
d. RNA polymerase II; synthesizing rRNA and tRNA
e. RNA polymerase I; synthesizing rRNA
, E
What does HAT do to affect gene expression?
a. HAT deacetylates lysine on the histone tail, allowing for interactions between
nucleosomes
b. HAT acetylates lysine on the histone tail, reducing interactions between
nucleosomes
c. HAT deacetylates lysine on the histone tail, reducing interactions between
nucleosomes
d. HAT acetylates lysine on the histone tail, allowing for interactions between
nucleosomes
e. None of the above
B
. Identify the following DNA-binding domain:
SEE IMAGE
a. Helix-turn-helix
b. Classical zinc finger
c. Nuclear receptor zinc finger
d. Leucine zipper
e. Helix-loop-helix
B
Remember that a classical zinc finger motif is characterized by a
Zn coordinated by 2 cys and 2 his amino acid residues.
1. Where in the cell are you likely to see molecules like
this?
SEE IMAGE
I. Nucleus
II. Cytoplasm
III. Plasma membrane
a. I only
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