MB (ASCP) TEST ATCUAL EXAM 300 QUESTIONS AND CORRECT (100% VERIFIED ANSWERS)
9 views 0 purchase
Course
MB
Institution
MB
Predominant form of DNA -Correct Answer Right-handed B
DNA and histone interaction is due to what type of bond? -Correct Answer Ionic
What stains to the minor groove of DNA duplex? -Correct Answer SYBR Green
What is the most abundant type of RNA? -Correct Answer Ribosomal RNA (rRNA)
Wha...
MB (ASCP) TEST 2023-2024 ATCUAL
EXAM 300 QUESTIONS AND CORRECT
(100% VERIFIED ANSWERS)
Predominant form of DNA -Correct Answer ✔Right-handed B
DNA and histone interaction is due to what type of bond? -Correct Answer ✔Ionic
What stains to the minor groove of DNA duplex? -Correct Answer ✔SYBR Green
What is the most abundant type of RNA? -Correct Answer ✔Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? -Correct Answer ✔Genome and mRNA
What increases half-life of mRNA? -Correct Answer ✔5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -Correct Answer
✔Euchromatin
What is the most common type of eukaryotic DNA? -Correct Answer ✔Satellite DNA
What specimen has the most DNA according to molecular weight? -Correct Answer
✔White Blood Cells
What amino acids compose histones? -Correct Answer ✔Lysine and Arginine
Amino acid that all proteins start with: -Correct Answer ✔Methionine
What protects the DNA strand during replication? -Correct Answer ✔Single-stranded
binding protein
Leading Strand -Correct Answer ✔3' to 5' template
Lagging Strand -Correct Answer ✔5' to 3' template
What DNA strand is being copied in transcription? -Correct Answer ✔Antisense Strand
First step after newly formed mRNA is made? -Correct Answer ✔Addition of poly(A) tail
MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
, MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
Every time the chromosome is copied it would become shorter if not for what enzyme? -
Correct Answer ✔Telomerase
What polymerase has repair activity? -Correct Answer ✔Beta/Epsilon
What polymerase acts on leading strand? -Correct Answer ✔Delta
What polymerase acts on lagging strand? -Correct Answer ✔Alpha
Which polymerase is used for mitochondrial replication and repair? -Correct Answer
✔Gamma
What polymerase binds the 5' end to 3' end of dsDNA? -Correct Answer
✔Topoisomerase
Which RNA polymerase is mainly for transcription? -Correct Answer ✔RNA Pol II
Which polymerase is involved with mRNA synthesis? -Correct Answer ✔RNA Pol II
What inactivates RNAses? -Correct Answer ✔Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -Correct Answer
✔Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? -Correct Answer
✔Restriction Enzyme Type II
What is necessary to make cDNA? -Correct Answer ✔Reverse transcriptase
Number of SNPs in a human genome? -Correct Answer ✔11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? -Correct Answer ✔3
billion base pairs
The polymerization of acrylamide requires: -Correct Answer ✔Catalyst (APS or TEMED)
or Light activation
How does agarose gel pore size relate to concentration? -Correct Answer ✔Inversely
proportional
What is denatured during a southern blot? -Correct Answer ✔Hydrogen bonds (inter-
strand bonds)
MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
, MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
Melting temperature formula -Correct Answer ✔Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? -Correct Answer ✔5' G
Proper GC content of primer -Correct Answer ✔35-65%
Inhibitors of PCR -Correct Answer ✔Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Bromophenol Blue
Order to add to a PCR reaction: -Correct Answer ✔Master mix > patient sample >
positive control > negative control
Pre Area -Correct Answer ✔Positive Pressure
Post Area -Correct Answer ✔Negative Pressure
Which PCR requires two steps for specificity? -Correct Answer ✔Nested PCR
Which microorganism is detected by branched DNA amplification? -Correct Answer
✔DNA or RNA
Which technique uses RNA, antibodies, and DNA? -Correct Answer ✔Hybrid Capture
Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation -Correct
Answer ✔Bisulfite DNA
What tests are isothermal? -Correct Answer ✔NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? -Correct Answer ✔AML
T-cell gene rearrangement is seen in what malignancy? -Correct Answer ✔Sezary
Syndrome
Which translocation causes Acute Premyelocytic Leukemia? -Correct Answer ✔t 15;17
Ewing Sarcoma -Correct Answer ✔t 11;22
Synovial Sarcoma -Correct Answer ✔t X;18
Follicular Lymphoma -Correct Answer ✔t 14;18
MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller PossibleA. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.99. You're not tied to anything after your purchase.
