Exam (elaborations)
BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass
- Course
- Institution
BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass
[Show more]
Content preview
BIOS 280 Lab Quiz 2; Questions and Answers 100% Passthe first step in the gram-stain process is to apply ____ stain to a heat-fixed bacterial smear;
the stain is then rinsed off with _____ Correct Ans-crystal violet
water
the second step in the gram-stain process is that the ____ solution is added to the slide; this
acts as a ____, which binds the crystal violet dye to the peptidoglycan layer in the cell wall
Correct Ans-iodine
mordant
the third step in the gram-stain process is that the bacterial smear is ____ with alcohol; any
dye not firmly fixed into the cell wall will wash out; then safranin, a ____, is applied to the
smear Correct Ans-decolorized
counterstain
gram-positive organisms will retain the crystal violet in their thick cell walls with the help of
the mordant and appear ____ Correct Ans-purple
,BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass
the alcohol removes the purple stain from gram-negative organisms, and the colorless cells
take up the safranin counterstain, which appear ____ Correct Ans-pink
____-fixing prevents the bacteria from washing off the slide Correct Ans-heat
the first step in heat-fixing the bacteria is to place 1-2 loopfuls of distilled water on a slide; be
sure to use an ____ loop; aseptically transfer a small amount of bacterial culture from a plate;
mix the culture with the distilled water on a slide; spread the liquid evenly over the slide
Correct Ans-incinerated
the second step in heat-fixing the bacteria is to allow the slide to ____-dry; it is dry when
there is no visible moisture or wetness on the slide Correct Ans-air
the third step in heat-fixing the bacteria is to heat-fix the slide by either leaving the slide on
the slide warmer for ____ minutes, or holding the slide (with a clothespin) against the outside
of a turned-on incinerator for ____ minutes Correct Ans-10
1-1.5
after the slide is heat-fixed, the slide is ready for ____ Correct Ans-staining
, BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass
the first step to perform a gram-stain is to place the slide on the staining rack over the sink;
you may use a clothespin to hold the slide; add 2-3 drops of ____ stain to the slide for about
30 seconds Correct Ans-crystal violet
the second step to perform a gram-stain is to rinse the slide with a small amount of distilled
____ Correct Ans-water
the third step to perform a gram-stain is to add 2-3 drops of Gram's ____ for 30 seconds; rinse
with distilled water Correct Ans-iodine
the fifth step to perform a gram-stain is to hold the slide at a 45 degree angle over the sink;
rinse with ____ until no more purple dye runs off; rinse with distilled water Correct Ans-
alcohol
the seventh step to perform a gram-stain is to add 2-3 drops of ____ for 30 seconds; rinse
with distilled water; carefully (gentle) blot the slide dry with ____ paper Correct Ans-
safranin
bibulous
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller GOLDTUTORS. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
79223 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now