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MCB 301: Biochemical Tests Questions and Answers | 100% Pass

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MCB 301: Biochemical Tests Questions and Answers | 100% Pass what medium is both selective and differential? - Answer️️ -MacConkey what two components of MacConkey agar inhibit growth of most gram-positive bacteria? - Answer️️ -bile salt and crystal violet what type of medium does not ...

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  • September 18, 2024
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MCB 301: Biochemical Tests Questions
and Answers | 100% Pass

what medium is both selective and differential? - Answer✔️✔️-MacConkey


what two components of MacConkey agar inhibit growth of most gram-positive

bacteria? - Answer✔️✔️-bile salt and crystal violet

what type of medium does not inhibit the growth of bacteria, but differentiates them

based on some visible growth characteristic (ex. colony color)? - Answer✔️✔️-differential

components of MacConkey agar - Answer✔️✔️-lactose, a fermentable carbohydrate, and

the pH indicator neutral red

indicator pH of MacConkey - Answer✔️✔️-6.8 (turns red below)

colonies turn this color on the MacConkey plate if they are unable to ferment lactose -

Answer✔️✔️-colorless or yellow

the MacConkey medium differentiates between which two types of bacteria? -

Answer✔️✔️-lactose-fermenting bacteria and lactose non-fermenters

Score MacConkey plate: Growth occurred and all colonies are noticeably pinkish red.

Acid has been produced - Answer✔️✔️-A

Score MacConkey plate: Growth occurred. Most colonies are colorless, but some look a

little pink/red - Answer✔️✔️-(A)

, TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024
Score MacConkey plate: Growth occurred and colonies are colorless. No acid has been

produced - Answer✔️✔️-0+

Score MacConkey plate: No growth occurred. Bile salts and crystal violet in the medium

inhibited growth of the organism - Answer✔️✔️-0-

Score Simmon's citrate agar: growth and/or any blue color change in the medium -

Answer✔️✔️-+

Score Simmon's citrate agar: no growth, no color change - Answer✔️✔️--

an organism that scored positive for the simmons citrate test is able to do what? -

Answer✔️✔️-transport citrate across the cell membrane and metabolize

Initial step in reduction of nitrate - Answer✔️✔️-reduction of nitrate (NO3-) TO nitrite

(NO2-)

What products are made from further reduction of nitrite? - Answer✔️✔️-N2, NH3

(ammonia), N2O (nitrous oxide), hydroxylamine

Growth in the presence of oxygen will do what to nitrate reduction? - Answer✔️✔️-

decrease or eliminate nitrate reduction

bacterial oxidation of the citrate anion to carbon dioxide by the TCA cycle releases the

__________ ______, which diplaces the ammonium ion - Answer✔️✔️-sodium cation

what molecule forms in the simmon's citrate medium which raises the pH and turns the

BTB indicator blue? - Answer✔️✔️-ammonium hydroxide

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