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PMCOL 371 Final Exam Study Guide.

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PMCOL 371 Final Exam Study Guide. Orthodromic Response - answerstimulate presynaptic fibers, record postsynaptic response. NPY inhibits Glu release (synaptically mediated action potentials) Antidromic Response - answerStimulate postsynaptic axons, observe cell body response Sharp Microelectrode...

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  • September 17, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • PMCOL 371
  • PMCOL 371
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PMCOL 371 Final Exam Study Guide.


Orthodromic Response - answer✔stimulate presynaptic fibers, record postsynaptic response.
NPY inhibits Glu release (synaptically mediated action potentials)

Antidromic Response - answer✔Stimulate postsynaptic axons, observe cell body response

Sharp Microelectrode - answer✔fine glass tip filled with strong salt. DC coupled amplifier.
Sub-threshold activity.

Wheatstone Bridge - answer✔balances resistances of pipette and bridge to neutralize effect of
pipette resistance. Initial response reflects only cell membrane properties.

Cell-autonomous Bursting - answer✔rhythmically depolarize even after isolation

Network Based Burst - answer✔Two tonic or no activity cells if active generate rhythm due to
mutual antagonist synaptic inhibition (inhibitory synapses)

Command Neuron - answer✔Tonic firing neuron, sensory input to get a not intrinsically
rhythmic circuit going

Trigger Neuron - answer✔Trigger rhythmicity of network.

Snail CPG - answer✔RPeD1, VD4, IP3I

IP31 - answer✔activation of VI/J

RPeD1 - answer✔Destroy CB, CPG fin for awhile. All signaling in dendrite and axons, only
ITM is shown, no LTM.

STG - answer✔CPG, STG, STNS

Gastric Mill CPG - answer✔Gpn, agn, lpgn, dgn
bursting irregular (except gpn), phasic relationship between them

Pyloric CPG - answer✔Pdn, avn, lpn, pyn, myn (AB, PD, LP, PY)
faster then mill, regular bursting with phasic relationships
AB and PD connected by gap junctions. Depolarization in AB/PD lead to LP hyperpolarization.

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LPG - answer✔regular fast bursting with pyloric activity and gastric silence
slow rhythm when gastric active indicative of DG cell activity

Apamin and Motor CPG - answer✔block AHP K/Ca, prolong duration, perturbs regularity of
antagonistic nerves in SC.

Mammal CPG - answer✔In vivo slower, require 5-HT, NMDA, DA. Rostral CPG stronger than
caudal, synaptic excitatory drive.

Breathing CPG - answer✔preBotC: inspiratory
pFRG: expiratory

pFRG - answer✔short pre-inspiratory activity, silent during inspiratory, strong post-inspiratory
activity.

preBotC - answer✔slow bursting -57, tonic past -47.

Breathing Currents - answer✔INa (slow inactivating), activate IKCa (AHP), ongong tonic
excitatory drive restarts breathing with positive Glu feedback.

Protein Kinases - answer✔CaMKII and PCK: activation induce LTP
MAPK: activated by LTP, inhibit late gene expression
PKA and Tyrosine Kinase: specific pathways

Feedback Somatic Inhibition - answer✔output axon excites interneuron that feedback and
inhibit CB

Feedforward Somatic Inhibition - answer✔Input axon excites inhibitory interneuron prior to
pyramidal neuron inhibiting CB. Curtail early excitation.

Feedforward Dendritic Inhibition - answer✔Input axon excites inhibitory interneuron after
pyramidal neuron inhibiting dendrite. GABAb slow activating stops activity eventually.

Feedback Dendritic Excitation - answer✔2nd Pyramidal neuron feedback onto 1st exciting the
dendrites

Feedforward Dendritic Excitation - answer✔1st pyramidal neuron feed forward onto 2nd
causing a second AP as it beats GIRK.

Non-linear Summation - answer✔more than additive. non-NMDA activation creates stronger
NMDA excitation

ChR2 - answer✔Na and Ca, 473nm

VChR1 - answer✔Na and Ca, 530-570nm

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