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(MB) ASCP PRACTICE EXAM QUESTIONS AND ANSWERS
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(MB) ASCP PRACTICE EXAM QUESTIONS AND ANSWERS
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(MB) ASCP PRACTICE EXAMQUESTIONS AND ANSWERS
Which0of0the0following0is0not0a0component0of0a0nucleotide?
Phosphate0group
Anti-codon
Ribose0sugar
Nitrogen0base0-0ans--✔✔Anti-codon
According0to0Chargaff's0rule0of0base0pairing,0adenine0pairs0with:0-0ans--✔✔Thymine
What0genes0would0be0screened0in0a0breast0cancer0panel?0-0ans--
✔✔HER2,0ERBB2,0BRCA1
Next0Generation0Sequencing0uses:0-0ans--✔✔Short0sequence0reads
Purines0and0pyrimidines0differ0from0each0other0in0that:0-0ans--
✔✔Purines0have0two0rings;0pyrimidines0have0one0ring
The0purines0are:
Cytosine0and0uracil
Adenine0and0thymine
Thymine0and0cytosine
Adenine0and0guanine0-0ans--✔✔Adenine0and0guanine
What0is0the0rate0of0mutation0per0round0of0DNA0replication?
10in01,0000base0pairs
10in010,0000base0pairs
10in01,000,0000base0pairs
10in01,000,000,0000base0pairs0-0ans--✔✔10in01,000,000,0000base0pairs
The0rate0of0DNA0migration0through0an0agarose0gel0during0electrophoresis0does0not0depe
nd0on0which0of0the0following0factors?
Net0charge0of0the0molecule
Size0of0the0molecule
Shape0of0the0molecule
Nucleotide0sequence0of0the0molecule0-0ans--✔✔Nucleotide0sequence0of0the0molecule
,What0are0the0phases0in0a0qPCR0Amplification0Plot?
Initiation,0exponential,0plateau
Baseline,0exponential,0plateau
Baseline,0threshold,0exponential,0plateau
Baseline,0initiation,0threshold,0exponential,0plateau0-0ans--
✔✔Initiation,0exponential,0plateau
Find0the0palindrome0in0this0restriction0enzyme0site:05'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC0-0ans--✔✔3'-GAC
A0patient0with0impaired0judgment,0personality0changes,0signs0of0abnormal0body0moveme
nts0and0depression0comes0to0the0physician's0office0for0a0follow-
up0visit.0The0physician0suspects0a0single-
gene0disorder0may0be0the0cause0of0those0clinical0manifestations.0A0blood0specimen0was0
then0sent0to0your0clinical0laboratory0for0mutation0screening0in0the0Huntington0gene.0Testi
ng0with0standard0PCR0indicates0that0patient0has0Huntington0Disease,0HD.0Which0of0the0f
ollowing0would0be0consistent0with0this0diagnosis?
250CAG0repeats0in0the0Huntington0gene
850CAG0repeats0in0the0Huntington0gene
250CGA0repeats0in0the0Huntington0gene
850CGA0repeats0in0the0Huntington0gene0-0ans--
✔✔850CAG0repeats0in0the0Huntington0gene
Which0two0HPV0types0are0responsible0for0most0cases0of0cervical0cancer?
160and018
310and059
160and058
440and0590-0ans--✔✔160and018
Replication0forks,0known0as0origins0of0DNA0replication,0are0created0by0this0enzyme:
Ligase
Taq0Polymerase
Primase
Helicase0-0ans--✔✔Helicase
Mutation0in0what0gene0is0associated0with0Fragile0X0syndrome?0-0ans--✔✔FMR1
Mantle0cell0lymphoma0(MCL)0is0caused0by0what0translocation?0-0ans--✔✔t(11;14)
This0polymerase0is0involved0in0"initiation0of0DNA0replication0and0has0primase0activity":0-
0ans--✔✔Pol0α
, Its0discovery0shed0light0on0why0there0is0simultaneous,0though0not0continuous,0synthesis0
of0DNA0on0both0leading0and0lagging0strands0of0DNA:
Klenow0fragment0of0DNA0polymerase
Okazaki0fragments
Sanger0fragments
RNA0fragments0-0ans--✔✔Okazaki0fragments
What0gene0is0measured0following0treatment0with0imatinib0(Gleevec)?
FLT3
BCR/Abl
Jak2
MAPK0-0ans--✔✔BCR/Abl
What0is0the0rate0of0mammalian0DNA0replication?
5000nucleotides0per0second
1000nucleotides0per0second
500nucleotides0per0second
100nucleotides0per0second0-0ans--✔✔500nucleotides0per0second
This0polymerase0is0involved0in0"replicates0mitochondrial0DNA":0-0ans--✔✔Pol0γ
A0patient0with0impaired0judgment,0personality0changes,0signs0of0abnormal0body0moveme
nts0and0depression0comes0to0the0physician's0office0for0a0follow-
up0visit.0The0physician0suspects0a0single-
gene0disorder0may0be0the0cause0of0those0clinical0manifestations.0A0blood0specimen0was0
then0sent0to0your0clinical0laboratory0for0mutation0screening0in0the0Huntingtin0gene.0Which
0of0these0methods0would0best0accomplish0this0task?
Methylation-specific0PCR
Standard0PCR
PFGE
RAPD0PCR0-0ans--✔✔Standard0PCR
Which0of0the0following0storage0options0is0optimal0for0storing0isolated0DNA0for0a0period0gr
eater0than0seven0years?
22-25ºC
2-8ºC
-20ºC
-70ºC0-0ans--✔✔-70ºC
Consider0a0hypothetical0mutation0involving0gene0X.0Let's0say0you0amplify0a0specific0exon
,0say0exon011,0of0that0gene0then0you0cut0it0with0restriction0enzyme0W.0In0a0person0withou
t0the0mutation,0cutting0the0gene0with0restriction0enzyme0W0generates0two0fragments0of0si
zes,01000bp0and02500bp.0Suppose0a0C>T0mutation0in0gene0X0deletes0a0restriction0site,0yi
elding0a0fragment0of03500bp.0You0would0expect0a0heterozygous0person0for0gene0X0to0hav
e0these0fragments0on0a0restriction0gel:
+/+0=03500bp;02500bp;01000bp;
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