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Exam (elaborations)

MLS ASCP EXAM REVIEW QUESTIONS AND ANSWERS GRADED A+

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MLS ASCP EXAM REVIEW QUESTIONS AND ANSWERS GRADED A+...

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  • September 16, 2024
  • 57
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • mls ascp
  • MLS ASCP
  • MLS ASCP
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MLS ASCP EXAM REVIEW QUESTIONS AND
ANSWERS GRADED A+

What tests are preformed on donor blood?

ABO, Rh (anti-D), Antibody screen (Rh, Kell, Kidd, Duffy), serologic tests (syphillis), viral
diseases (HCY-RNA, HBsAg, Anti-HBc)

One unit of RBCs increases hgb and hct by how much?

Hgb: 1g

Hct: 3%

For leukoreduced RBCs the final WBC count must be?

<5x10^6

Dyglycerated cells are protected from low temps with what concentration of glycerol
agent?

40%

One unit of platelets increases platelet count by

5,000-10,000

One unit of apheresed platelets should increase the platelet count _________.

20,000-60,000

what do irradiated blood components do?

prevent GVHD by innactivating T-cells

Plasma should be stored at

-18C

RBCs should be stored at

1-6C

platelets should be stored at

22C

,Group O has the greatest amount of what antigen?

H

Group A1B has the least amount of what antigen?

H

Type O blood has __ antigens

Neither A nor B

Type AB has what antigens?

A and B antigens

Type A blood has what antigens?

A antigens

Type B blood has what antigens?

B antigens

Type O blood has what antibodies?

anti-A and anti-B antibodies

Type AB blood has what antibodies?

no antibodies

Type A blood has what antibodies?

anti-B antibodies

Type B blood has what antibodies?

anti-A antibodies

Type O blood can receive

O only

Type O blood can donate to

A, B, AB, O (universal donor)

Type AB blood can receive

A, B, AB, O (universal recipient)

Type AB blood can donate to

,AB only

Type A blood can receive

types A and O

Type A blood can donate to

Type A and Type AB

Type B blood can donate to

Type B and Type AB

Type B blood can receive

Type B and Type O

unknown RBCs + anti-A = agglutination indicating the cells DO have the the antigen in
the antisera. This is an example of?

Forward blood typing

unknown plasma + A1 cells = agglutination, indicating the serum HAS the antibody to the
antigen on the red cells. This is an example of?

reverse blood typing

unknown plasma + B cells = agglutination, indicating the serum HAS the antibody to the
antigen on the red cells. What is the patient's reverse type?

negative

unknown RBCs + anti-B = agglutination indicating the cells DO have the the antigen in
the antisera. What is the patient's forward type?

B

A patient exhibits agglutination in the tube containing anti-A + RBCs and in the tube
containing plasma + B cells. What is the patients blood type?

A-

What are clinically significant antibodies?

IgG antibodies including: Kell, Kidd, Duffy, ABO, Rh, (S,s,U), and Vel

91% of people are negative for what IgG antibody?

Kell (K)

What IgM antibody is negative on infant cells but positive on adult?

, Anti-I

What IgM antibody is NOT present on chord cells?

LeA and LeB

Is IAT in-vivo or in-vitro?

in vitro

Is DAT in-vivo or in-vitro?

in vivo

True or false: IAT detects antibodies in plasma

true

True or false: False positives in IATs are caused by inadequate washing, under
centrifugation, and delay in adding antiglobulin reagent

false

false NEGATIVES are caused by those things

True or False: a positive IAT indicates the blood is NOT compatible.

true

Adding LISS to the serum and red cells enhances:

allows for stronger reactions and speeds up test.

Adding enzymes (bromelin, ficin, papin, and trypsin) increases reactivity of what
antibodies?

Rh, Kidd, and Lewis

Adding enzymes (bromelin, ficin, papin, and trypsin) destroys what antibodies?

M, N, S, Fya & Fyb

PEG increases the strength of agglutination reactions by:

removing water

Antiglobulin in DAT reacts with what?

IgG antibodies/complement

What sample type is preferred for DAT's because it prevents complement activation?

EDTA

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