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mip 302 practical Exam Questions with Verified Answers
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mip 302 practical Exam Questions withVerified Answers
why do endospore + cells stain with a clear "hole" in the center during gram stains? -
✔✔the stains can't penetrate the spore in the cell's center without heat
application and gram stains don't use heat
dilution general equation - ✔✔(# sample parts) / (total # parts)
total # parts = # sample parts + # diluent parts
PFU/mL equation - ✔✔# phage = (PFU/mL of original sample) x dilution x
volume plated
when testing chemotherapeutic agents, why is it necessary to match the bacterial
inoculum against a 0.5 McFarland standard? - ✔✔this way, we can ensure
approx. the same # of bacterial cells are inoculated on the same plate for each test
, what is the range of # of bacterial colonies that are considered countable? -
✔✔30-300
colony shapes - ✔✔Circular, irregular, rhizoid (spider), punctiform (stipple)
colony textures - ✔✔rough, dry, wrinkled, shiny, dull
colony margins - ✔✔entire, erose, filamentous, lobate
colony colors - ✔✔translucent, yellow, white, cream, etc
what should be included when asked to characterize a colony? - ✔✔colony
shape, texture, margin, and color
Why is broth not used as an isolation medium? - ✔✔Can't differentiate
between types of bacteria
should TSA slant be used as an isolation medium? - ✔✔probably not - the
surface area is generally too small to differentiate between types of bacteria
What color do gram positive bacteria stain? - ✔✔purple
What color do gram negative bacteria stain? - ✔✔pink
Why do gram positive bacteria stain the color they do? - ✔✔g + bacteria stain
purple because of their thick cell wall that contains peptidoglycan and teichoic acids
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