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MIP 300 Study Guide Questions with Verified Answers | A+ Guaranteed
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MIP 300 Study Guide Questions withVerified Answers | A+ Guaranteed
Dark Field Microscope - ✔✔-Background is dark, organism is
light -Light is refracted by organism
-in the field, dont have to heat fix or stain
ex.Syphilis
phase contrast microscope - ✔✔-Shades of grey to show contrast in cell
components -slight differences in refractive indexes into light intensity -Motility
because no fixing or staining
fluorescence microscopy - ✔✔-Uses UV light to excite fluorescent molecules
-Some cells take up the dye
-Common in studying viruses
Magnification - ✔✔Objective X eyepiece
,Resolution - ✔✔the ability of the lenses to distinguish between objects that are
close together
Best resolution is .2um
human cells - ✔✔10-100 micrometers
Bacteria - ✔✔1 micrometer
Viruses - ✔✔.01-.1 micrometer
electron microscope - ✔✔-Resolution 1000x better than
light -Start to see viruses
Simple stain - ✔✔1 stain is used to increase microbes visibility
Differential stain - ✔✔Divides microbes into different categories depending
on structural differences
Gram stain - ✔✔-Separates bacteria by the type of cell wall they have
-most common stain
Pos=purple neg=pink
acid fast stain - ✔✔Identifies bacteria that are neither gram + or
- that hav a thick waxy layer(mycolic acid) around them Pos=hot
pink Neg=blue
, endospore stain - ✔✔Identifies spore-producing
bacteria Green spores pink vegetative cells
sporangium - ✔✔spore capsule in which haploid spores are produced by meiosis
flagella stain - ✔✔identifies bacteria that possess flagella used for motility
Coccus - ✔✔A spherical bacterium.
Diplococci - ✔✔cocci growing in pairs
Streptococci - ✔✔bacteria that form a chain
Staphylococci - ✔✔in random planes grape like clusters
Tetrads - ✔✔in 2 planes 4 cell groups
sarcina - ✔✔in three planes cube of 8 cell groups
Bacillus - ✔✔Rod shaped bacteria
coccobacillus - ✔✔short round rod, resemble cocci
diplobacillus - ✔✔bacillus growing in pairs
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