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Portage Microbiology BIOD171 Exam 5 || with Complete Solutions.

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  • Portage Microbiology BIOD171

Define the concept of universal precautions. correct answers Universal precautions means any and all samples, whether known or unknown, are to be treated as potentially hazardous (or pathogenic) materials. List at least 3 observations a researcher would be sure to note while assessing an unknown...

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  • September 12, 2024
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  • 2024/2025
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  • Portage Microbiology BIOD171
  • Portage Microbiology BIOD171
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Portage Microbiology BIOD171 Exam 5 || with Complete
Solutions.
Define the concept of universal precautions. correct answers Universal precautions means any
and all samples, whether known or unknown, are to be treated as potentially hazardous (or
pathogenic) materials.

List at least 3 observations a researcher would be sure to note while assessing an unknown
microbial sample. correct answers A lab researcher would be certain to note:

Size and shape
Any observable motility
Gram status (positive or negative)
The presence of any chemical reactions
Changes in color localized to the organism or the surrounding media
Capture (or draw) images of any of the characteristics described above

While observing an unknown sample of limited amounts, a researcher must determine the
following observations: (1) the presence of any motility and (2) its Gram status using the same
sample—the liquid sample cannot be divided. Which would you determine first and why? correct
answers You must determine motility before determining the Gram status. Motility requires a
wet mount, while Gram staining requires heat fixing the sample. If one were to begin with the
Gram stain the heat fixation process would kill the organism, making any observations regarding
motility impossible. The correct approach would be to place the liquid culture on a glass slide
and determine its motility status. Next, the same liquid culture can be heat fixed and Gram
stained.

A facultative anaerobe is a microorganism capable of growth under what conditions? correct
answers A facultative anaerobe is capable of growth under aerobic (with oxygen) and anaerobic
(without oxygen) conditions.

As Streptococcus is catalase negative would it thrive or die in the presence of peroxides? Why?
correct answers Streptococcus is catalse negative which means it cannot break down peroxides
and if any peroxides are present it would die.

Streptococcus is most often streaked onto: correct answers Blood agar


Strep is often cultured on Blood agar plates to determine its hemolytic properties, which aids in
the classification (and differentiation) process.

True or False. The Lancefield groups are used to subdivide antigenic groups of gamma-
hemolytic Streptococcus. correct answers False

The Lancefield groupings are used to subdivide beta-hemolytic Strep.

, The distinctions for Lancefield subgroupings lie in its: (select all that apply)

Catalase activity
Hemolytic activity
Carbohydrate composition of antigens
All of the above correct answers Carbohydrate composition of antigens


Carbohydrate composition of antigens present in the cell wall determines the Lancefield
groupings (A, B, C, etc). Note, ALL strep under Lancefield groupings are (by definition) catalase
negative and beta-hemolytic. Thus, answers A and C cannot be used to subdivide streptococcal
species.

Left untreated, strep throat can progress to ______ , which displays _____ hemolytic activity.
correct answers Rheumatic fever; beta

True or False. Under most circumstances, staphylococcus can be found in ~30% of human
population where it remains pathogenic. correct answers false; While staphylococcus can be
found in ~30% of the human population, it remains non-symptomatic.

When a bacterium neither harms nor benefits from the host from which it obtains nutrients, it is
referred to as being ___________. correct answers Commensal

Attachment correct answers pathogen finds a specific target tissue and adhere

evasion correct answers pathogen masks its own antigens to hide from the host's immune system

entry correct answers utilization of a portal to gain access to host's tissues

Match the following diseases with their respective symptoms:
1. folliculitis
2. scalded-skin syndrome
3. impetigo
4. conjunctivitis
5. ophthalmia neonatorum

A. Childhood skin disease near mouth/nose
B. Infection of thin, transparent scleral tissue
C. Infection occurs at time of birth
D. Pus-filled lesions on skin or hair
E. Ruptured pustules; treated with penicillin correct answers 1. D
2. E
3. A
4. B
5. C

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