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MCBM Enzymes Questions with 100% Correct Answers
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MCBM Enzymes Questions with 100% Correct Answers
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1 of 68Term
What is the difference between the lock and key model and the
induced fit model of enzyme-substrate binding?
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Describes the reaction velocity and how it varies with different concentrations of
enzyme and substrate
, Some enzymes are only active after a certain chunk of their sequence is cleaved
by proteolytic enzymes. Ex: blood clotting and digestive enzymes (trypsinogen -
-> trypsin)
Lock and key suggests that substrates fit directly into the active site and
are acted upon. Whereas the induced fit model suggests that the enzyme
alters its own conformation once the substrate initially binds to create an
even better fit
Reaction velocity increases with increasing temperature until the enzyme begins
to denature. The optimal temperature for most of our enzymes is 35-40 C
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2 of 68
Term
What does Km represent in the MM equation?
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The substrate concentration at which the velocity of the reaction is half of
the max velocity
A low affinity of the enzyme for the substrate. The enzyme will hesitate before
grabbing the substrate, at least until the substrate concentration has increased
dramatically
, They are molecules that destroy a part of the enzyme that are essential for
function (usually through a covalent interaction)
It can help determine Km and Vmax, as well as determine the mechanism of
action of enzyme inhibitors
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3 of 68
Term
What is the lineweaver-burk plot?
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The change in free energy required
Proteins that can bind to enzymes
to convert a molecule into its
to either inhibit or stimulate activity
transition state of a reaction.
It is the reciprocal of the plot Usually a cleft or crevice on a larger
provided by the MM equation 3D protein structure
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, 4 of 68
Term
What is an apoenzyme?
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A complete, catalytically active The protein part of a
enzyme holoenzyme
An inactive precursor to an enzyme Metal ions or vitamin derivatives
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5 of 68
Term
Describe catalysis with acids an bases
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Reactive groups are brought in close proximity to one another to induce the
transition state
Interactions between the metal and the substrate helps to orient and stabilize
the transition state of the substrate
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