Exam (elaborations)
ACS Biochemistry First Semester Questions and Answers 100% Accurate 2024
- Course
- Institution
ACS Biochemistry First Semester
[Show more]
Content preview
ACS Biochemistry First SemesterBuffer Order and Henderson Hasselbach - answer Water, Buffer, Salt, Base
pH = pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
Ramachandran plots - answerΦ = N-R
ψ = C-R
optimal angle to prevent steric hinderance
Protein structure of a helix, B sheet, reverse turn - answer a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - answer3.6
Super secondary structure - answerhelix turn helix
parallel vs. antiparallel - answerparallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - answerpH at which a particular molecule carries no net electrical
charge.
Protein Separation Techniques (ALL) - answerHomogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG
Protein Separation Techniques (H) - answerHomogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - answerSalting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - answerAffinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
, Protein Separation Techniques (I) - answerion exchange
separation based on charge; + proteins stick to - beads
Protein Separation Techniques (SG) - answerSize Exclusion/Gel Filtration
separation based on size
Cuts (protein separation techniques) - answercut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)
Dialysis - answerprotein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Electrophoresis - answergels - move by size with electric current
1. Isoelectric focusing - purely pH
2. SDS page
pH separation is by pI and charge in 2d
Protein sequencing (who cuts after what) - answerCyanogen bromide = methionine
trypsin = K, and R
chymotrypsin = F, M, L, W, Y
IP - answerImmunoprecipitation with proteins
1. add antibodies specific to protein of interest to lysed cells
2. add antibody binding protein on beads
3. centrifuge and protein is on bead with antibodies
4. gel elect
CO-IP - answerobserve protein protein interaction
Messelson Stalh - answerRATSHIT.
Western Blotting - answerrun gel - transfer to sheet - stained with radioactive antibody -
autoradiogram - exposed antibody
ELISA - indirect and sandwich - answerEnzyme linked immunosorbant assay
1.) indirect - antigen coated well - bind persons antibody - bind antigen with enzyme -
bind substrate = rate of color
2.) Sandwich - antibody coated well - bind person antigen - bind 2nd antibody with
enzyme - bind substrate = rate of color
Mass spec - answeridentify proteins by size
MALDI - answermatrix assisted laser desorption ionization (BIG)
ESI - answerelectric spray ionization - charged molecules - time of flight detector
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller julianah420. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77254 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now