MICROB 201 EXAM 3 PRACTICE QUESTIONS AND ANSWERS (100% PASS)
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Course
MICROB 201/ 202
Institution
MICROB 201/ 202
MICROB 201 EXAM 3 PRACTICE QUESTIONS AND
ANSWERS (100% PASS)
housekeeping genes - Answer️️ -genes that are expressed/transcribed all the time;
are constitutively expressed (transcribed in an ongoing manner)
regulatory proteins - Answer️️ -act as "molecular switches" that control gene
e...
MICROB 201 EXAM 3 PRACTICE QUESTIONS AND
ANSWERS (100% PASS)
housekeeping genes - Answer✔️✔️-genes that are expressed/transcribed all the time;
are constitutively expressed (transcribed in an ongoing manner)
regulatory proteins - Answer✔️✔️-act as "molecular switches" that control gene
expression; bind to regulatory DNA sequences located next to coding region of
genes
- activator proteins activate gene expression
- repressor proteins inhibit gene expression
operon - Answer✔️✔️-a gene or group of genes under control of a single promoter
and its associated regulatory DNA sequences
how many genes are in the lac operon of e. coli? - Answer✔️✔️-three genes
lac operon - Answer✔️✔️-consists of a set of genes that encode a polycistronic
mRNA that specifies production of enzymes required for using lactose as a carbon
source
- expressed in the presence of lactose and the absence of glucose
β-galactose - Answer✔️✔️-an enzyme encoded by the lacZ gene of lac operon that
converts lactose into glucose and galactose
negative regulation of lac operon - Answer✔️✔️-lac operon is regulated by a
repressor protein; protein binds to operator thereby repressing transcription
- allolactose will bind to lac repressor to prevent it from binding to operator
- transcription may now occur; β-Gal can now break down lactose
positive regulation of lac operon - Answer✔️✔️-requires an activator protein c'AMP
Receptor Protein (CRP) to interact with RNA polymerase to permit transcription
- in the absence of the activator, RNA polymerase cannot associate with the
promoter
what does expression of the lac operon depend on? - Answer✔️✔️-- presence of
inducer allolactose, a lactose derivative
- lactose to inactivate the repressor
- c'AMP to activate the activator
recombinant DNA (rDNA) - Answer✔️✔️-uses enzymes to ligate or join relatively
small DNA fragments from one source (such as a gene) to form a DNA cloning
vector
DNA cloning vector - Answer✔️✔️-small piece of DNA in which foreign DNA can
be inserted into and maintained in an organism
purpose of rDNA - Answer✔️✔️-to isolate a single gene or DNA fragment from one
source and replicate it in high quantities, often in another organism
restriction enzymes - Answer✔️✔️-used in rDNA to recognize and cleave double-
stranded DNA at palindromic sequences
palindromic sequences - Answer✔️✔️-nucleic acid sequences where the one strand
matches its complementary strand when read in the same direction
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