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BGM3039 - L16 Exam Latest Update
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BGM3039 - L16 Exam LatestUpdate
what is surgical resection margin - Answer residual cancer at edge of resection
= critical prognostic factor in cancer surgeruy
-defined by sight and feel
how does surgical resection treat cancer - Answer 40% treated following removal of
tumour margin with clearances
what are the current intra-operative imaging solutions - Answer paraffin embedded
sectioning
forensic sectioning
how does paraffin embedded sectioning work - Answer -water replaced by paraffin in
tissues
-microtome slices and sections viewed under microscope
-H+E staining for cell morphology
what is a disadvantage of paraffin embedded sectioning - Answer not immediate (2
days)
how does frozen sectioning work - Answer freeze with cryostat and sectioned
what are the disadvantages of freeze sectioning - Answer -poor quality section (not easy
to cut)
-bloated cell morphology (water expands)
-poor stained section
what is Stokes shift - Answer the difference in nm between peal excitation and peak
emission wavelengths
-each fluorophore has distinct stokes shift
-high stokes shift prevents interference
how does fluorescence-guided surgery work - Answer light to locally injected
fluorophore and imaged under microscope during surgery = visualise tumour
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