Name the different organism morphologies - ANSWERSCoccus
Coccobacillus
Vibrio
Bacillus
Spirillum
Spirochete
Name the bacterial arrangements - ANSWERSCoccus
diplococcus
staphylococci
streptococci
sarcina
tetrad
Describe a Filamentous Rod - ANSWERSA long string of bacillus bacteria
Describe a Fusiform Rod - ANSWERSBacteria that is pointed on the ends
Describe Pleomorphic Rods - ANSWERSDifferent lengths and shapes of the same bacteria
What is the melting and liquid temperatures of agar? - ANSWERSPolysaccharide melts at 100C and stays
liquid until 45-50C.
Nutrient Agar - ANSWERSThe most basic growth media for bacteria. Examples: TSA and Nutrient Agars
, Enriched Media - ANSWERSSupplemented with various substances to encourage growth of fastidious
organisms. Examples: BAP and Chocolate.
Enrichment Broths - ANSWERSEnhance the growth of specific MOs while inhibiting the growth of others.
Examples: GN, Selenite, LIM and Trans-Vag broths.
Nonselective Media - ANSWERSNo antimicrobial substances and allows growth of most organisms.
Examples: BAP and Chocolate.
Selective Media - ANSWERSContains antimicrobial substances which allows the growth of specific
bacteria while inhibiting the growth of others. Examples: PEA, CNA, MAC, EMB
Capnophilic - ANSWERSIncreased CO2 (5-10% CO2) Example: Haemophilus influenza.
Humidophilic - ANSWERSIncreased humidity (70-80%)
Incinerating - ANSWERSBurns and physically destroys MOs
Intermittent Boiling - ANSWERSKills all MOs (3-30 minute intervals of boiling at 100C and cooling kills
endospores)
Autoclaving - ANSWERSSteam under pressure (15 psi at 121C for 15 minutes)
Dry Heat - ANSWERSHot air oven (160C for 2 hours)
Irradiation - ANSWERSDestroys and distorts nucleic acids of MOs. Examples: UV lamps, Gamma
radiation, Electron beam radiation.
Filtration - ANSWERSPhysical removal of bacterial cells from liquids, gases and air. Example: HEPA filters
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