Summary of chapter 13: Enzymes of Clinical Chemistry by Bishop
14 views 0 purchase
Course
Clinical Chemistry
Institution
Saint Louis University
Book
Clinical Chemistry: Principles, Techniques, and Correlations, Enhanced Edition
Providing an organized, summarized, and easy to read discussion of Chapter 13: Enzymes on Clinical Chemistry by Bishop along with highlights on important and must know information.
ENZYMOLOGY
PURPOSE TISSUE DX SIGNIFICANCE ASSAY SOURCES OF
SOURCES ERROR
CREATININE KINASE
● Associated with ATP ● Skeletal muscle ● Elevated in disorders of cardiac and skeletal ● CK catalyzes both forward ● Hemolysis
regeneration in ● Heart muscle muscle (myocardial infarction [MI], and reverse reactions - Erythrocytes are
contractile or ● Brain tissue rhabdomyolysis, and muscular dystrophy) involving phosphorylation of virtually devoid of
transport systems. ● Bladder, placenta, ● Sensitive indicator of AMI and muscular creatine or ADP. CK; but are rich in
● Involved in the gastrointestinal dystrophy, particularly the Duchenne type. ● Change in absorbance at 340 AK activity, which
storage of tract, thyroid, ● Early diagnostic tool to identify patients with nm reacts with ADP to
high-energy creatine uterus, kidney, Vibrio vulnificus infections and ectopic ● Reverse reaction proposed by produce ATP,
phosphate. lung, prostate, pregnancies Oliver and modified by which is then
● Isoenzymes: spleen, liver, and ● Seen in: Rosalki is the most commonly available to
○ CK-BB (brain type) pancreas ○ Strokes, seizures, nerve degeneration, and performed method participate in
○ CK-MB (hybrid central nervous system shock, damage to ● Reverse is faster than forward assay reaction,
type) BBB ● Optimal pH for causing falsely
○ CK-MM (muscle ● Increased in: reverse reaction= 6.8; elevated levels.
type) ○ Hypothyroidism forward reaction = 9.0. ● Hemoglobin
○ Malignant hyperpyrexia ● Unstable releases sufficient
○ Reye’s syndrome ● Inactivation can be partially AK to exhaust the
● MM form reversed by the addition of AK inhibitors in the
- Majority of CK activity in heart muscle N-acetylcysteine, reagent.
- Major isoenzyme fraction found in striated mercaptoethanol, thioglycerol, ● Vary with muscle
muscle and normal serum or dithiothreitol mass and, therefore,
- Hypothyroidism ● Reverse Rxn= Oliver Rosalki may depend on
- Predominant isoenzyme ● Forward rxn= Tanzer Givarg gender, race, degree
of physical
conditioning, and
age.
● Mild to strenuous
activity may
contribute to
elevated CK levels,
as may
intramuscular
injections.
● CK is inactivated by
light.
● Rapidly inactivated
because of oxidation
of sulfhydryl groups.
,
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller larryshawn. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.49. You're not tied to anything after your purchase.
