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Microbiology Lab Exerc ise 2 Microscopy and Staining Rxam Questions and Complete Solutions Graded A+ $14.49   Add to cart

Exam (elaborations)

Microbiology Lab Exerc ise 2 Microscopy and Staining Rxam Questions and Complete Solutions Graded A+

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  • Course
  • Microbiology 2104
  • Institution
  • Microbiology 2104

Microbiology Lab Exerc ise 2 Microscopy and Staining Rxam Questions and Complete Solutions Graded A+

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  • August 26, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Microbiology 2104
  • Microbiology 2104
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Microbiology Lab
Exercise 2 Microscopy
and Staining Exam
Questions and Complete
Solutions Graded A+
Denning [Date] [Course title]

, staining - Answer: can be used to make it easier to view bacteria using a bright field microscope and
provides contrast



what is staining useful for identifying? - Answer: morphology, size and arrangement



what are the 4 different types of staining? - Answer: simple, differential, structural and negative



what type of staining is this exercise? - Answer: simple



differential staining - Answer: differentiate bacteria or microbes based on certain characteristics



cationic dyes - Answer: basic dyes, positively charged chromophore



what are cationic dyes attracted to? - Answer: net negative charge of bacterial cells and stain the cells



what are two examples of cationic dyes? - Answer: methylene blue and crystal violet



anionic dyes - Answer: acidic dyes, negatively charged chromophore



what are anionic dyes repelled by? - Answer: the negative charge of the bacterial cell, the background of
the cell is dyed



what is an example of an anionic dye? - Answer: nigrosin



negative stain - Answer: - uses an anionic dye, so the stain does not penetrate the cell

- the cells are repelled by the negatively charged dye and have transparent cells against a colored
background



what is a negative stain good for? - Answer: examining morphology and size of bacterial cells because
heat is not used, so distortion of the bacterial cells is minimized

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