CELL BIOLOGY LAB PRACTICAL
MATERIAL 3111L UNCC QUESTIONS
AND ANSWERS
dilutions - Correct Answer - x x x x x
molarity calculations - Correct Answer -
x x x x x x
macromolecules: which reagent is used for each macromolecule - Correct Answer - x x x x x x x x x x x
lipids, carbs and proteins
x x x x
lipids- Sudan IV test. sudan IV solution dissolves lipids and stains them red.
x x x x x x x x x x x x x
carbs- benedicts test (detects reducing sugars) and IKI test (reacts with many starch like
x x x x x x x x x x x x x
polysaccharides, but not simple carbs)
x x x x x x
proteins- lowry rxn produces a bluish black color. carbonate-copper reagent and folin
x x x x x x x x x x x
ciocalteau reagent. Biuret reagent turns light blue to purple. Biuret reacts with
x x x x x x x x x x x x
polypeptides and not amino acids.
x x x x x
how will you make 20 ml of 20% salt solution - Correct Answer - 16ml of water and add
x x x x x x x x x x x x x x x x x x
salt till it reaches 20 ml
x x x x x x
describe how will you create 1M tris solution
x x x x x x x
(tris- 121.14g/mol) - Correct Answer - add 121.14 g to 1 L of water
x x x x x x x x x x x x x x
which test will detect proteins? - Correct Answer - biuret test
x x x x x x x x x x
what proteins help to compact DNA? - Correct Answer - histones. 8 protein subunits
x x x x x x x x x x x x x
come together to make one histone core.
x x x x x x x
you need 0.1 M DTT and the stockroom has 1M DTT. How will you make 100 mL of 0.1
x x x x x x x x x x x x x x x x x x
M DTT? - Correct Answer - add 10 mL of 1M DTT to 90mL of water
x x x x x x x x x x x x x x x x
Which device will measure 163 uL most accurately? - Correct Answer - micropipette
x x x x x x x x x x x x
which device will measure 3.8 mL most accurately? - Correct Answer - pipette
x x x x x x x x x x x x
convert 94 mm to nm - Correct Answer - 94 x 10^6
x x x x x x x x x x x
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