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Electrophoresis – Complete Study Set With Solutions
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Electrophoresis – Complete Study Set With Solutions
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Electrophoresis – Complete Study Set With SolutionsPositively charged ions are called __ and migrate towards the cathode Right
Ans - cations (=charge)
Negatively changed ions are called __ and migrate towards the anode Right
Ans - anode (+charge)
What does the force of the charged particle depend on? Right Ans - electric
field (V)
charge on the particle (Q)
What two properties of the particle affects the velocity? Right Ans - charge
frictional coefficient
What is an isoelectric point? Right Ans - pH at which net charge of a
molecule is zero
What is the net charge of a molecule in a pH lower than the pI? Right Ans -
positive
What is the net charge of a molecule in a pH higher than the pI? Right Ans -
negative
How does a molecule become positive in a pH lower than pI? Right Ans -
hydrogen ions are donated from the buffer
What buffer is used in electrophoretic solutions? Right Ans - sodium
barbital
tris-barbital
What is added to the environment to provide constant electrical environment
so overall movement of protein is constant? Right Ans - Buffer and
electrolytes
What is zeta potential? Right Ans - potential that exists between the fixed
ions and the associated cloud of ions
, What is electroendosmosis? Right Ans - movement of buffer solutes and
solvent relative to support matrix when electric field is applied
Describe the electroendosmosis effect Right Ans - positive ions cluster
around the negative sites forming an ionic cloud
Which media cannot be used to see gamma globulins because of their weak
charge? Right Ans - cellulose acetate
paper
agar gel
What causes wavy electrophoretic patterns? Right Ans - high voltage which
increases heat production
increases migration and denatures proteins
What is wick flow? Right Ans - flow of buffer from each reservoir is equal
but is opposite in direction
What is the function of buffers? Right Ans - carry current
fix the pH
Why does increased ionic strength decrease migration rate? Right Ans -
thicker ionic cloud hinders movement
What is the advantage of higher ionic strength ? Right Ans - increased
resolution/sharper bands
What is the disadvantage of higher ionic strength? Right Ans - increased
heat production
What is molecular sieving? Right Ans - molecules small enough to pass
through the pores of the media
Which transparent medium can be read by a densitometer? Right Ans -
cellulose acetate
agarose
polyacrylamide
starch gel
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