BIO 3300 Exam 1 Questions with Correct Answers
Prokaryotic Cells Correct Answer-Most diverse and numerous cells on
earth. These types of cells are divided into two domains- bacteria and
archaea.
Eukaryotic Cells Correct Answer-Contain membrane bound organelles-
including a nucleus (nucleus contains the most DNA).
Light Microscopy Correct Answer-Magnify cells up to 1000x and
resolves detail as small as 0.2 um. Resolution depends on the light
source. Can view living cells.
Fluorescence Microscopy Correct Answer-Cells are fluorescently dyed.
Similar to light microscopy, except the light passes through two filters.
Filter one only allows light that excites the particular fluorescent dye
through. Filter two blocks out the light and only lets through the
wavelengths that excite the fluorescent dye. Can view living cells.
Confocal Microscopy Correct Answer-A specialized type of fluorescent
microscope that builds up an image by scanning the specimen with a
laser beam. The beam is focused at a single point at a specific depth.
Can view living cells.
TEM Microscopy Correct Answer-A beam of electrons is used as the
light source and passed through three magnetic coils that function as the
lenses in a vacuum. Specimen has to be thin and chemically fixed into
plastic. Sample is stained with salts of uranium and led. Cannot use live
specimens.
, SEM Microscopy Correct Answer-Specimen is coated with a very thin
film of heavy metal, and is scanned by a beam of electrons focused on
the specimen by magnetic coils that act as lenses. You only get the
surface picture due to the specimen deflecting the electrons. Cannot use
live specimens.
Nucleus Correct Answer-Holds DNA.
Mitochondria Correct Answer-Responsible for cellular respiration.
Produces ATP. Most likely evolved from engulfed bacteria.
Chloroplasts Correct Answer-Capture energy of sunlight. Evolved from
engulfed photosynthetic bacteria.
Smooth Endoplasmic Reticulum Correct Answer-Involved in synthesis
of sterols and other molecules.
Rough Endoplasmic Reticulum Correct Answer-Has ribosomes and
plays a role in protein synthesis.
Golgi Apparatus Correct Answer-Packages and transports proteins.
Plasma Membrane Correct Answer-Import by endocytosis and export by
exocytosis.
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