Exam (elaborations)
BIO 2060 SLCC Questions and Answers 2024
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Exam of 39 pages for the course SLCC at SLCC (BIO 2060 SLCC)
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BIO 2060 SLCCWhat organisms are used at SLCC? (BSL levels 1-4) – answer BSL 1
Change clothing, shower when leaving, and decontaminate everything when exiting.
Wear a full-body protective, pressurized suit with a designated air supply. Stay in a
separate building with a separate air supply. – answer BSL - 4
What BSL level are the microbiology labs at SLCC? Why? – answer BSL 2 compliant
Commercial sterilization is different from sterilization used in medical and laboratory
settings. ____________ kills pathogens that normally cause spoilage and foodborne
disease but allows nonpathogenic organisms to survive. - answer Commercial
sterilization
___________ are chemicals that effectively kill all microbes and viruses with sufficient
exposure time and can also kill endospores – answer Sterilants
Aseptic technique refers to - answer prevents/infection contamination• Combination of
protocols that collectively maintain sterility
A designated area that is kept free of vegetative microbes, endospores, and viruses is
called __________. - answerSterile Field
Inanimate items, such as doorknobs, toys, or towels, which may harbor microbes and
aid in disease transmission, are called.... - answerfomites
What does "BSLs" refer to? - answerbiological safety levels
BSL, BSL-1, requires the ___________ precautions because it applies to situations with
the _____________ risk for microbial infection. - answerfewest /
lowest
Agents classified as BSL-2 include those that pose a ____________ risk to laboratory
workers and the community.
a. high
b. moderate
c. low - answermoderate
Which of the following types of medical items requires sterilization?
a. needles
,b. bed linens
c. respiratory masks
d. blood pressure cuffs - answerNeedles require sterilization
Which of the following is suitable for use on tissues for microbial control to prevent
infection?
a. disinfectant
b. antiseptic
c. sterilant
d. water - answerAntiseptic
Which biosafety level is appropriate for research with microbes or infectious agents that
pose moderate risk to laboratory workers and the community, and are typically
indigenous?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4 - answerBSL-2
Which of the following best describes a microbial control protocol that inhibits the growth
of molds and yeast?
a. bacteriostatic
b. fungicidal
c. bactericidal
d. fungistatic - answerfungistatic
The decimal reduction time refers to the amount of time it takes to which of the
following?
a. reduce a microbial population by 10%
b. reduce a microbial population by 0.1%
c. reduce a microbial population by 90%
d. completely eliminate a microbial population - answerreduce a microbial population by
90%.
A medical item that comes into contact with intact skin and does not penetrate sterile
tissues or come into contact with mucous membranes is called a(n) ________ item. -
answernoncritical
The goal of ________ ________ protocols is to rid canned produce of Clostridium
botulinum endospores. - answercommercial sterilization
True or False: Sanitization leaves an object free of microbes. - answerFalse
Medical surveillance. Extra vaccines. Respirator use. Use of biological safety cabinet,
always. Hands-free sink. 2 sets of self-closing/locking doors. Directional airflow:
a. BSL - 1
,b. BSL - 2
c. BSL - 3
d. BSL - 4 - answerBSL - 3
Restricted access. Required PPE. Biological safety cabinets ("hoods"). Self-closing
doors. Eyewash station. Autoclave:
a. BSL - 1
b. BSL - 2
c. BSL - 3
d. BSL - 4 - answerBSL - 2
The standard aseptic technique is used on an open lab bench with PPE, a sink for hand
washing, and doors.
a. BSL - 1
b. BSL - 2
c. BSL - 3
d. BSL - 4 - answerBSL - 1
Which BSL poses a high risk of aerosol-transmitted infections, which are frequently fatal
without treatment or vaccines?
a. BSL - 1
b. BSL - 2
c. BSL - 3
d. BSL - 4 - answerBSL - 4
Mycobacterium tuberculosis is an example of a BSL ______
a. BSL - 1
b. BSL - 2
c. BSL - 3
d. BSL - 4 - answerBSL - 3
Which is an example of a BSL - 2?
a. Ebola and Marburg viruses
b. Staphylococcus aureus
c. Mycobacterium tuberculosis
d. Nonpathogenic strains of Escherichia coli - answerStaphylococcus aureus
Agents classified as BSL-2 include those that pose moderate risk to laboratory workers
and the community and are typically "_____________," meaning that they are
commonly found in that geographical area. - answerindigenous
What are some factors used to determine the BSL necessary for working with a specific
pathogen? - answer-Infectivity
-Ease of transmission
-potential disease severity --type of work being done
, Chemicals that can be used to achieve sterilization are called _________. -
answersterilants
Failure to practice aseptic technique during many types of clinical procedures may
introduce microbes to the patient's body and put the patient at risk for _____________,
a systemic inflammatory response to an infection that results in high fever, increased
heart and respiratory rates, shock, and, possibly, death. - answersepsis
Medical procedures that carry risk of contamination must be performed in a
____________ ___________. - answersterile field
True or False: temperatures low enough to preserve food quality but high enough to
destroy common pathogens responsible for food poisoning, such as C. botulinum, is an
example of sterilization. The same procedures used in medical and laboratory settings. -
answerFalse
True or False: Disinfection inactivates most microbes on the surface of a fomite using
antimicrobial chemicals or heat. A disinfected item is considered sterile. - answerFalse
Chlorine bleach, phenols (e.g., Lysol), vinegar are all examples of :
a. Sterilization
b. Disinfection
c. Sanitization - answerDisinfection
Match:
1. Disinfection ________
2. Sanitization ________
3. Sterilziation________
a. Completely eliminates all vegetative cells, endospores, and viruses from an
inanimate item
b. Reduces microbial load of an inanimate item to safe public health levels through
application of heat or antimicrobial chemicals
c. Reduces or destroys microbial load of an inanimate item through application of heat
or antimicrobial chemicals - answer1. Disinfection: c. Reduces or destroys microbial
load of an inanimate item through application of heat or antimicrobial chemicals
2. Sanitization: b. Reduces microbial load of an inanimate item to safe public health
levels through application of heat or antimicrobial chemicals
3. Sterilziation: a. Completely eliminates all vegetative cells, endospores, and viruses
from an inanimate item
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