ACS BIOCHEMISTRY FINAL EXAM
2024 ACTUAL EXAM COMPLETE
150 QUESTIONS WITH DETAILED
VERIFIED ANSWERS (100%
CORRECT ANSWERS) / ALREADY
GRADED A+
what factors favor the T-state in hemoglobin? -
....ANSWER...low pH, high CO2 and high 2,3 BPG at the
level of the tissues favor the taut form, which has low
oxygen affinity and releases oxygen in the tissues
what factors favor the R-state in hemoglobin? -
....ANSWER...high pH, low CO2, or low 2,3 BPG favors
the relaxed form which can better bind oxygen.
how does oxygen bind in hemoglobin? -
....ANSWER...oxygen binds to the heme component of the
protein hemoglobin in red blood cells
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what are lipids with glycosidic linkage? -
....ANSWER...glycerolipids, where one or more sugar
residues are attached to glycerol via a glycosidic linkage
What is the pI of an amino acid - ....ANSWER...point at
which the net charge is zero; point of least solubility in
water
How to calculate the pI - ....ANSWER...(1) determine the
highest positive charge possible
(2) list all of the pKas in order from smallest to largest
(3) start in the space before the first pKa and jump to the
next space as many times as there are positive charges
(4) average the pKa above and below the space you end up
in
What is the charge of an amino acid when (a) pH<2 (b)
pH=7 and (c) pH>9 - ....ANSWER...(a) positive
(b) neutral (zwitterion)
(c) negative
Difference between a cation and anion exchange column -
....ANSWER...cation exchange column-attracts positively
charged molecules
State the central dogma of genetics - ....ANSWER...DNA
can self replicate via translation
DNA is coded into RNA via transcription
RNA is made into proteins via translation
Structure of a nucleotide - ....ANSWER...5 carbon sugar,
phosphate group, and a nitrogenous base
Name the monomer of nucleic acids -
....ANSWER...nucleotides
Describe the relative stability of DNA and RNA -
....ANSWER...DNA is more stable than RNA because
DNA lacks the 2' hydroxyl group; since DNA only has a 3'
hydroxyl group, the phosphodiester bonds formed are
specific and rigid; also, the C-H bond at the 2' carbon in
deoxyribose is much less reactive than the C-OH bond in
ribose
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