ACS BIOCHEMISTRY EXAM 2024
ACTUAL EXAM COMPLETE 250
QUESTIONS WITH DETAILED
VERIFIED ANSWERS (100%
CORRECT ANSWERS) / ALREADY
GRADED A+
what are the corresponding enzymes in glycolysis and
gluconeogenesis - ....ANSWER...pyruvate kinase is the
corresponding enzyme in glycolysis for PEPCK and
pyruvate carboxylase
what metabolic conditions can activate gluconeogenesis? -
....ANSWER...low F6BP; high citrate;
epinephrine/glucagon
what are the products of the pentose phosphate pathway? -
....ANSWER...NADPH, ribose
,List all the enzymes that use OAA as a substrate -
....ANSWER...Pyruvate carboxylase, citrate synthase,
malate dehydrogenase
what reaction is catalyzed by the coenzyme TPP
(thiamine/B1)? - ....ANSWER...Oxidative decarboxylation
What reaction is catalyzed by the coenzyme
tetrahydrofolate (folic acid)? - ....ANSWER...One carbon
group transfer
what reaction is catalyzed by the coenzyme pyridoxal-5-
phosphate (b6) - ....ANSWER...Amino group transfer
what reaction is catalyzed by the coenzyme Cobalamin
)B12) - ....ANSWER...Alkylation
What reaction is catalyzed by the coenzyme lipoamide -
....ANSWER...Transfer of an acetyl group to CoA
What is the chemiosmotic theory? -
....ANSWER...Electron transport generates a proton
gradient across the inner membrane due to H+ being
pumped out; the energy released (proton motive force)
,when H+ returns to the matrix is used to power ATP
synthesis
What are ketogenic substrates degraded to? -
....ANSWER...acetyl CoA or ketone bodies
What are glucogenic substrates degraded to? -
....ANSWER...pyruvate, alphaketoglutarate, succinyl CoA,
fumarate, OAA
what is the equation to determine the number of cycles of
B-oxidation of a saturated fatty acid? - ....ANSWER...n/2-
1
Describe what metabolic processes occur in the muscle
and liver in the glucose-alanine cyvles during branched
chain amino acid catabolism - ....ANSWER...branched
chain amino acids are degraded in the muscle and the NH3
is transported to the liver in the form of Ala. The liver uses
the Ala for gluconeogenesis and the glucose is transported
to the muscle for glycolysis.
what is an antagonist? - ....ANSWER...binds to a receptor
and prevents a response by keeping agonist from binding
, what is an antagonist - ....ANSWER...binds a receptor and
causes a response
Is transverse or lateral diffusion in a lipid bilayer least
likely to occur? - ....ANSWER...transverse
If triglycerides and phospholipids are separated using
TLC, what spot is more soluble (will be at the top)? -
....ANSWER...tryglyceride; phospholipid will be less
soluble so will not travel as far
In a gel filtration column, what proteins elute the quickest?
- ....ANSWER...the larger proteins that have a higher kDa
elute first
what amino acid sequence is most likely to be in the core
of a globular protein? why? - ....ANSWER...ITFWLI; no
charged amino acids and highest percent nonpolar
an example of a sticky restriction enzyme? -
....ANSWER...A CGTGA
an example of a blunt restriction enzyme? -
....ANSWER...ACG TGA
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