MCB4304 Prep Exam 1 Questions & Answers 2024/2025
Operon - ANSWERSGroup of genes transcribed as a single polycistronic message. Controlled by a regulatory protein. Only in Prokaryotes.
Regulon - ANSWERSGroup of genes transcribed as separate units. Controlled by a single regulatory protein.
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Operon - ANSWERSGroup of genes transcribed as a single polycistronic message. Controlled by a
regulatory protein. Only in Prokaryotes.
Regulon - ANSWERSGroup of genes transcribed as separate units. Controlled by a single regulatory
protein.
lactose permease - ANSWERSlactose transporter. brings lactose into the cell
B-galactosidase - ANSWERScleaves glycosidic bond. Breaking Lactose into Glucose and Galactose
lacZ - ANSWERSbeta-galactosidase
lacY - ANSWERSlactose permease
Negative regulation of transcription (inducible) - ANSWERSRepressor binds to repressor binding site,
blocking transcription. Until an inducer binds repressor, together leave DNA, allowing transcription
Negative Regulation (repressible) - ANSWERSAporepressor must bind with co-factor in order to be able
to bind repressor binding site, then blocking transcription.
Positive Regulation - ANSWERSan activator protein binds activator binding site to recruit RNA Polymerase
(Transcriptase)
lacI - ANSWERSencodes the lac repressor
Complementation - ANSWERSGene added is the same as Mutation
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