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BIOS-242 Lab Practical Review 100% Solved
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BIOS-242 Lab Practical Review 100% Solved
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BIOS-242 Lab Practical Review 100% Solved_____ ____ uses H2O2 to determine if the bacteria can use oxygen or not. If the test is
positive (produces bubbles) the organism is Staphylococci, if the test is negative (no
bubbles) the organisms is streptococci - ANS-catalase test
_____ ______ spread of pathogen via air, drinking water, and food, as well as bodily
fluids being handles outside the body - ANS-vehicle transmission
_____ _______- stain purple, have thick layer of peptidoglycan - ANS-gram positive
_____ ____- essentially no hemolysis at all- bacteria have no effect on the red blood
cells, and there is no change to the color of the medium (ex. normal flora) - ANS-
gamma hemolysis
______ _____ (_______)- for bacteria with mycolic acid - ANS-acid fast
(mycobacterium)
______ _____ exhibits color change when a certain kind of microorganisms grows on
them giving us information about the type of microbe growing there - ANS-differential
media
______ ______ _____(____)- key ingredient is sheep blood; tested on bacteria
inoculated from a patient's throat swab to test for the presence of streptococci - ANS-
Blood Agar Plate (BAP)
______ ______ (________)- carbolfuschin is primary dye- heat to drive stain inside -
ANS-acid fast (mycobacterium)
______ ______ spread of infection from one host to another by direct contact, indirect
contact or respiratory droplets - ANS-contact transmission
______ ______ the primary optical lenses on a microscope. - ANS-optical lens
______ _______ (pathogenic) is mannitol fermenter and turns MSA a bright yellow color
- ANS-staphylococcus aureus
______ _____- stain pink, contains much thinner layer of peptidoglycan - ANS-gram
negative
______ _____- the bacteria's hemolytic enzymes completely break the blood cells- the
pattern results in the media displaying a clear halos around bacterial colonies (ex.
streptococcus pyogenes) - ANS-beta hemolysis
, ______ _____- the bacterial enzymes only partially break down the blood cells -the
results in the media showing a yellowish/greenish/brownish discoloration (like a bruise)
around the colony, indicating incomplete hemolysis (ex. normal flora) - ANS-alpha
hemolysis
______ _____: selective medium used to cultivate gram + organisms. Active ingredient:
phenylethyl alcohol, which inhibits growth of gram- organisms by interfering with DNA
synthesis - ANS-PEA plate
______ _____/ ____ ______ _____- the organism is spread out on the plate to give
lawn of growth after incubation. Filter paper soaked in antibiotic solution is placed onto
the plate prior to incubation. During incubation the drug will diffuse out and , if it is
effective, will give a clear zone of where growth is prevented - ANS-Kirby Bauer / disk
diffusion test
______ (________)-background is dark, primary stain is eosin - ANS-capsule
(klebsiella)
______ (______)- thin hair like, hard to stain - ANS-flagella (e.coli)
______ connects to the base and supports the microscope head. It is also used to carry
the microscope - ANS-neck/arm
______ is clusters of cocci - ANS-staphylcocci
______ is clusters of rods - ANS-staphylobacilli
_______ ____ allows growth of only certain types of microbes and inhibits the growth of
other types of microbes - ANS-selective media
_______ ______(normal flora) does not ferment mannitol and the naturally orange-pink
color of the agar does not change - ANS-staphylococcus epidermidis
_______ _____- key ingredients are neutral red (pH indicator), lactose (disaccharide),
crystal violet, and bile salts - ANS-MacConkey Agar
_______ _____-regular agar plate with added nutrients to promote growth - ANS-
nutrient agar plate
_______ ____: selective and differential medium used to isolate fecal coliforms. Eosin Y
and methylene blue are pH indicator dyes which combine to form a dark purple
precipitate at low pH - ANS-EMB plate
_______ animals that transmit diseases from one host to another - ANS-vectors
_______ is long chains of bacillus - ANS-streptobacilli
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