Micro 260 Lab Pre Quizzes -Questions and Correct Answers (Expert verified) / Latest Update 2024/2025/100% Pass
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Course
Micro 260
Institution
Micro 260
Quiz 1-# 1[True or False?] It is acceptable to pick up a test tube by its cap. - FALSE Some
caps are VERY loose. If you pick up a test tube by its cap, and the cap is loose, the entire
glass test tube can fall to the floor and shatter. This would result in glass shards flying as
well as a potent...
Micro 260 Lab Pre Quizzes -Questions and Co rrect Answers (Expert verified ) / Latest Update 2024/2025/ 100% Pass Quiz 1 -# 1[True or False?] It is acceptable to pick up a test tube by its cap. - ✔✔FALSE Some caps are V ERY loose. If you pick up a test tube by its cap, and the cap is loose, the entire glass test tube can fall to the floor and shatter. This would result in glass shards flying as well as a potentially bio -hazardous situation (if there was a culture in the t ube). Quiz 1 -# 2[True or False?] Food and drinks are NEVER allowed in the laboratory. - ✔✔True Quiz 1 -# 3[True or False?] True Sandals or open -toed shoes are allowed in the lab. - ✔✔False Quiz 1 -# 4[True or False?] You must report all culture spills to your instructor prior to attempting clean -up. - ✔✔TRUE Quiz 1 -# 5[True or False?] Culture tubes must always remain upright in a test tube holder (i.e. test tube rack) or in your hand. - ✔✔TRUE Quiz 1 -# 6[True or False?] Microbes may be taken outside of the laboratory - ✔✔False Quiz 1 -# 7[True or False?] Eye protection (i.e. safety glasses) must be worn whenever you are working with liquid cultures or heating chemicals. - ✔✔True Quiz 1 -# 8 What is the proper sequence, from beginning to end, of the three things you must do every time you ENTER the microbiology laboratory? - ✔✔Put on lab coat... put on gloves... disinfect bench. Quiz 1 -# 9 What is the proper sequence, from beginning to end, o f the things you must do every time you are preparing to EXIT the microbiology laboratory? - ✔✔Disinfect bench... remove gloves (discard in trash)... remove lab coat (store in lab drawer)... wash hands Quiz 1 -# 10 What is the function of agar in culture m edia? - ✔✔solidifying agent Quiz 1 -# 11Microbes are ubiquitous. This means that they are... - ✔✔found virtually everywhere Quiz 1 -#12[True or False?] MOST microbes are pathogenic. - ✔✔FALSE Quiz 1 -# 13[True or False?] Used test tubes should be placed in the Biohazard bag for sterilization and disposal. - ✔✔False -...in the test tube basket WITH LABELS REMOVED Test tubes are NEVER placed in an autoclave bag (nor is anything that is glass or sharp enough to puncture a bag). Test tubes should always be place d in the designated BASKETS for autoclaving, washing, then re -use. Quiz 1 -#14 It is important to always wear ____________________________________________when heating chemicals, working with live cultures, and using stains. - ✔✔safety glasses, gloves, lab coat, and close -toed shoes Quiz 2 -# 1[True or False?] In order to calculate the TOTAL magnification of a specimen, the ocular lens magnification must be added to the magnification of the objective lens that is being used. - ✔✔FALSE The total magnification is calculated by MULTIPLYING the ocular lens magnification and the objective lens magnification. Quiz 2 -# 2The coarse focus knob on a compound light microscope may be used when the _________________ objective(s) is(are) being used to view a specimen. - ✔✔SCANNING POWER The coarse focus knob causes the stage to make large movements up and down. If the objective lens is very close to the specimen (and thus the stage), as we see with high power (400X) and oil immersion (1000X), adjusting focus with the COURSE focus knob can cause the objective lens to go right through the specimen! This will cause broken glass shards to fly and the microscope lenses to be scratched and permanently damaged! --> DON'T DO IT. **Use the coarse focus knob ONLY during your FIRST focusing step whe n the lowest power objective lens (scanning) is in place over the specimen. After that, use the FINE ADJUSTMENT KNOB -for every other objective lens.** Quiz 2 -# 3 Microscope transportation should always be done ........ - ✔✔with both hands Quiz 2 -# 4 Wha t type of paper can be used to clean oil off the microscope objective lens? (IMPORTANT NOTE: The oil immersion objective lens, 1000X, is the ONLY lens that should ever come in contact with oil!) - ✔✔lens paper ONLY Other types of paper will scratch and permanently damage the lenses. Quiz 2 -# 5 What component of a compound microscope functions to concentrate visible light into a narrow cone that is directed through the specimen, thus improving the function of the objective lenses? - ✔✔condenser Quiz 2 -# 6 What component of a compound microscope functions to adjust the diameter of light that goes through the condenser? This microscope component consists of thin overlapping metal leaves that can be moved to widen, or narrow, the diameter of light that ente rs the condenser. - ✔✔iris diaphragm Quiz 2 -# 7 What microscope component is adjusted in order to increase contrast? - ✔✔iris diaphragm Quiz 2 -# 8Higher power objective lenses magnify to a greater extent and typically require ___________ light intensity compared to lower power objective lenses. - ✔✔increased Quiz 2 -# 9 There is(are) _______ focus knob(s) on a compound microscope. - ✔✔two Quiz 2 -# 10 When a specimen is in focus on one objective lens (i.e. one magnification), it is almost in focus on the other objective lenses (higher magnifications) as well. This is because each compound microscopes is ____________. Therefore, once a specimen is in focus, only small movements with the fine focus knob are required as the operator moves from one objective lens to the next. - ✔✔parfocal
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