What method is used to stabilize proteins making it resistant to further damage? - ANSWER-Fixation
Name two methods of fixation? - ANSWER-Chemical reaction (formaldehyde) and physical (heat or dessication)
What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** - ANS...
Histotechnology Exam 1
Questions fully solved & updated
What method is used to stabilize proteins making it resistant to further damage? - ANSWER-Fixation
Name two methods of fixation? - ANSWER-Chemical reaction (formaldehyde) and physical (heat or dessication)
What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** - ANSWER-bacteria or mold
What factor causes autolysis if tissue is not fixed? **** - ANSWER-
enzyme attack
What are four functions of fixatives? - ANSWER-1. kills tissue to prevent putrefaction and autolysis
2. maintains proper relationship between intracellular and extracellular substances 3. preserves refractive indexes
4. enhances stains
What three organs contain the highest levels of enzymes? - ANSWER-
liver, kidney, and brain Histotechnology Exam 1
Questions fully solved & updated
True or False. You can over fix tissue? - ANSWER-True. Over fixation dries out the tissue and limits the quality. Can attempt steaming the sections to remedy over fixation.
If you receive a breast sample on a Friday night, what method must be performed to prevent exceeding the allowed formalin fixation time to perform the Her2 test? - ANSWER-Must place the tissue in 70% alcohol for one day and then place in formalin the following day.
How quickly does formalin penetrate tissue? - ANSWER-1 mm per hour
True or False. When fixing a liver specimen, the fixation time should be doubled? - ANSWER-True. The enzymes found in the cytoplasm need to be fixed well.
What is the name of a fixative that is combined with the protein molecule? Give an example. - ANSWER-additive fixative Ex. formaldehyde
What is the name of a fixative that removes water from tissue? Give two examples - ANSWER-nonadditive fixative. Ex. alcohol or acetone
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